SARS-CoV感染Vero E6细胞诱导细胞凋亡  

SARS-CoV Infection Induces Apoptosis of Vero E6

作　　者：鄢然[1] 沈超[1] 雷磊[1] 郑从义[1] 肖庚富[1] 郭德银[1] 朱应[1] 鄢慧民[1] 

机构地区：[1]武汉大学生命科学学院,湖北武汉430072

出　　处：《中国病毒学》2003年第6期541-543,共3页Virologica Sinica

基　　金：湖北省SARS科技攻关计划特别基金(2003AA305803);武汉大学SARS科技攻关特别基金

摘　　要：为了确定SARS冠状病毒(SARS-CoV)感染Vero E6是否引起细胞凋亡,我们利用细胞DNA琼脂糖电泳,感染细胞的间接荧光染色和Hoechst 33258细胞核染色,以及流式细胞仪分析等方法证明了SARS-CoV感染的Vero E6具有典型的凋亡细胞学和生物化学特征。实验证明具有细胞凋亡特征的所有细胞均为处于感染晚期的细胞。表现明显细胞病变(CPE)的细胞大多已经出现核质凝缩或形成凋亡小体进入细胞凋亡的过程。可以断定SARS-CoV感染Vero E6细胞诱发了细胞凋亡。To determine if SARS-associated coronavirus (SARS-CoV) induces apoptosis, we studied the SARS-CoV infected Vero E6 cells by agarose gel electrophoresis, indirect fluorescent staining for infection and Hoechst 33528 staining for nuclei, as well as by flow cytometry. It was found that SARS-CoV infected Vero E6 cells showed typical apoptosis characteristics. All the typical apoptotic cells were in late-infection period. Most of the cells with cytopathic effect (CPE) showed nuclei with condensed chromatin or fragmented into apoptotic bodies. It was suggested that SARS-CoV infection induces apoptosis of Vero E6.

关 键 词：SARS冠状病毒 细胞凋亡 VeroE6细胞 生物化学特征 

分 类 号：R373[医药卫生—病原生物学] Q255[医药卫生—基础医学]