东北地区新城疫流行株的分子生物学特性调查  

Epidemiological and phylogenetic analysis of NDV strains isolated in northeastern China

作　　者：崔尚金[1] 刘文斌[2] 李建伟[1] 王凯波[1] 李秀云 刘果 潘家鸣 张寰波 董玉光 

机构地区：[1]中国农业科学院哈尔滨兽医研究所疫病诊断与流行病学中心,黑龙江哈尔滨150001 [2]沈阳农业大学畜牧兽医学院,辽宁沈阳110161 [3]长春市畜禽防疫工作总站,吉林长春130062

出　　处：《中国兽医科技》2003年第12期26-31,共6页Chinese Journal of Veterinary Science and Technology

摘　　要：从东北地区发生新城疫 (ND)的病鸡、病鸽和产蛋下降鸡分离到 19株NDV ,对其融合蛋白 (F)基因 5 32bp或 2 80bp片段进行了克隆、测序 (在GenBank中的登录号为AY2 0 86 80～AY2 0 86 98) ,并对各株的F基因序列进行了比较。结果 ,各毒株之间的核苷酸同源性为 83.1%～10 0 % ,氨基酸同源性为 85 .5 %～ 10 0 % ;系统发育进化树分析表明 ,其中 2株与疫苗株V4同属基因Ⅰ型 ,为弱毒株 ;其余 17株为强毒株 ,其中 2株为基因Ⅵ型 ,其余为基因Ⅶ型。表明东北地区目前由 3种基因型的NDV毒株 (Ⅰ型、Ⅵ型、Ⅶ型 )所引发 ,并以基因Ⅶ型为主。另外 ,对其中的代表毒株APMV1/chicken/China/JL 11/ 0 2进行了免疫学试验 ,结果显示 ,LaSota疫苗的免疫保护效果不理想 ,可能是LaSota疫苗免疫鸡群发生ND的主要原因。19NDV strains were isolated from sick chickens and pigeons or egg drop chickens in northeastern China. These strains were studied by RT-PCR and the sequences of the region of 532 or 280 nucleotides covering the fusion (F) protein cleavage site were analyzed (GenBank accession No.AY208680-AY208698). Nucleotide and predicted amino acid similarities of 19 NDV were 83.11%-100% and 85.5%-100% respectively. Phylogenetic tree of the nucleotide sequence demonstrated that 2 strains are lentogenic strains and shared genetype Ⅰwith vaccine strain V4, 17 strains are velogenic and 2 strains of them were genetype Ⅵ and the others were genetype Ⅶ. At the same time, the representational strain APMV1/chicken/China/JL-11/02 was evaluated by trial of immunity. The result revealed that LaSota vaccine can not give good protection. This is probably important reason of immunity lose with LaSota.

关 键 词：东北地区 鸡 新城疫 流行株 分子生物学 

分 类 号：S858.31[农业科学—临床兽医学]