应用新城疫病毒强弱毒株RTPCR鉴别诊断技术检测多种禽类副黏病毒  

Application of the rapid differential diagnosis technique for Newcastle disease to detection of avian paramyxoviruses

作　　者：韦天超[1] 韦平[1] 杨宗维[1] 李莉萍[1] 左天荣[1] 李康然[1] 刘禄 甘甲忠 冯超 

机构地区：[1]广西大学养禽与禽病研究所,广西南宁530005 [2]广西出入境检验检疫局,广西南宁536028 [3]广西玉林市兽医站,广西玉林537000

出　　处：《中国兽医科技》2003年第12期44-47,共4页Chinese Journal of Veterinary Science and Technology

基　　金：广西自然科学基金资助项目 (桂科自 0 2 4 900 6 ) ;玉林市科研项目 (玉市科字 2 0 0 2 5 0 5 )

摘　　要：应用已建立的新城疫病毒 (NDV)强、弱毒株RT PCR快速鉴别诊断技术 ,对来自广西各地疑为禽副黏病毒血清 1型 (APMV 1)感染的 14 4份不同禽类的病料进行了检测 ,并用常规方法对RT PCR检测为阳性的部分病料进行了病毒的分离和鉴定。结果 ,从鸡、鸽、鹅、鸭、鹌鹑、珍珠鸡、孔雀、鸵鸟和画眉鸟 9种禽的病料样品中检测到APMV 1,阳性检出率为 6 4.5 8% (93/ 14 4 ) ;从 7种禽病料中分离到 2 9株APMV 1地方野毒株。部分分离株用NDV强、弱毒株快速鉴别技术鉴定属中、强毒株。The rapid differential diagnosis technique for detection of virulent and non-virulent NDVs was used to detect 144 clinical samples of avian paramyxovirus type 1 (APMV-1) suspect from different poultry species. Some positive samples were also diagnosed by the classical isolation and identification. The results showed that 64.58% of the samples from chickens, pigeons, geese, ducks, quail, peacock, thrush and ostrich respectively were positive of NDV. 29 Field strains of APMV-1 were isolated from 7 (avian) species origins and some were classified as mesogenic or velogenic strains of NDV by the rapid differential diagnosis technique. The results demonstrated that the developed technique of RT-PCR basis is a specific, sensitive, rapid and reliable for the diagnosis of APMV-1 infections in different poultry species origins and applicable in the laboratory diagnosis.

关 键 词：新城疫病毒 强弱毒株 RT-PCR鉴别 诊断技术 检测技术 禽类 副黏病毒 

分 类 号：S858.3[农业科学—临床兽医学]