基于线粒体12S和16S rRNA基因部分序列的角蟾亚科部分属种的系统发育关系  

Phylogenetic Relationships of Some Species and Genera in Megophryids Inferred from Partial Sequences of Mitochondrial 12S and 16S rRNA Genes

作　　者：江建平[1] 袁富蓉[2] 谢锋[1] 郑中华[1] 

机构地区：[1]中国科学院成都生物研究所,四川成都610041 [2]美国德克萨斯大学医学中心生理学系,圣安东尼奥782293900

出　　处：《Zoological Research》2003年第4期241-248,共8页动物学研究（英文）

基　　金：国家自然科学基金资助项目 ( 3 0 0 0 0 0 18) ;中国科学院生物科学与技术特别支持费资助项目 (STZ -0 1-19) ;四川省科技厅重点项目 ;香港野生动物保护基金会有限公司 (WCF)项目

摘　　要：测定了角蟾亚科 2属 8种 (亚种 )和外群 3种的线粒体 12S和 16SrRNA基因部分DNA序列 ,比对后序列长共 94 9bp ,其中变异位点数 32 0 ,简约位点数 2 0 6。邻接法和最大简约法分析的系统关系树一致表明内群为一单系群 ,其中腺角蟾首先与其他物种分开 ;沙坪角蟾与宽头短腿蟾聚为一支 ;余下的 5种 (亚种 )角蟾组成一支 ,其中小角蟾短肢亚种的广西种群和香港种群聚为一亚支 ,另一亚支包括峨眉角蟾、小角蟾指名亚种、尾凸角蟾和重庆武隆的角蟾种 ,后两种角蟾进化关系最近。本结果支持短肢角蟾为有效种 ,同时提示腺角蟾、沙坪角蟾与宽头短腿蟾可能隶属 3个不同的亚属或属。Fragments of mitochondrial 12 S and 16 S rRNA genes of 17 individuals were sequenced,including 8 species (subspecies) of 2 genera Megophrys and Brachytarsophrys of Megophrinae,and 3 species used as outgroup including the genera Bombia , Oreolalax and Scutiger .Results pairwised by Clustal W show that there are 949?bp in length with 320 variable sites including 206 parsimony sites.The average contents of T,C,A,and G are 25 6%,24 0%,29 8%,and 20 6%,respectively,and the ratio of transition to transvertion is 1 3.The phylogenetic relationships of these taxa were analyzed using programs of Neighbor Joining and Maximum Parsimony.The results show that the ingroup are clustered together as one clade,of which 3 individuals of M glandulosa were clustered together (BSP=100%) and firstly diverged from the other taxa (BSP=100%),3 individuals of B carinensis were clustered together (BSP=100%) as sister group of M shapingensis ,the rest 5 taxa formed together and included two groups,one consisting of two populations of M minor brachykolos from Longsheng of Guangxi and Hong Kong,the other containing M omeimontis,M minor minor,M caudoprocta and M sp from Wulong of Chongqing,the later two species are closely related.These results support M brachykolos as a valid species rather than a subspecies of M minor ,and suggest that M glandulosa,M shapingensis and B carinensis may belong to three different subgenera or genera.

关 键 词：角蟾亚科 角蟾属 短腿蟾属 无耳蟾属 RRNA基因 无尾类 

分 类 号：Q953[生物学—动物学]