蛋白质过瘤胃保护增强植物外啡肽对山羊生长代谢的调节作用  

Exorphins in the Plant that Regulate Growth Metabolism in Goats with Protein Bypass Rumen Protection

作　　者：孙镇平[1] 周业飞[1] 朱王飞[1] 华棣[1] 

机构地区：[1]扬州大学畜牧兽医学院,扬州225009

出　　处：《畜牧兽医学报》2004年第1期6-9,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：江苏省科技厅自然科学基金项目(BG97072)

摘　　要：采用过瘤胃保护技术使饲料中的小麦蛋白避开山羊瘤胃微生物破坏作用,进入皱胃和小肠降解,产生活性肽,增强对山羊生长代谢的调节作用。建立了蛙心体外循环系统,利用外啡肽特异阻断剂—纳洛酮,生物检测不同消化液中植物外啡肽的活性。结果表明:对照期和试验期相比24h采食青草量分别为4 65±0 231,6 413±0 039kg,差异极显著(P≤0 001);血清类胰岛素生长因子 1(IGF 1)分别为153 647±18 670,223 993±19 450ng/ml,差异极显著(P<0 01)。血清生长激素(GH)23 170±0 891,26 792±1 003ng/ml,差异极显著(P<0 01)。血清胰岛素18 103±1 035,23 983±1 600ng/ml,差异极显著(P<0 01)。血清氨基氮0 102±0 003,0 140±0 004mM/ml,差异极显著(P<0 001)。血清氨氮0 206±0 012,0 086±0 006mM/ml,差异极显著(P<0 001)。蛋白过瘤胃保护后的皱胃液以及未保护的瘤胃液能使心肌收缩力显著增强,加入纳洛酮后心肌收缩力都显著性逆转降低,从而证明这些消化液具有较强的外啡肽活性。表明蛋白质过瘤胃保护技术能够有效地增强植物外啡肽对生长代谢的调控作用。This paper has applied the technique of bypass rumen protection to prevent the wheat protein in forage by breakdown of microbial.Thus,it can enter into abomasum and small intestine,in which bioactive peptides are resolved and can play a regulation role in growth metabolism.The activity of plant exorphins in different digesta was determined with the system of frog heart circulation in vitro and with naloxone-specific inhibitor of exorphins.Comparing the contrast period with the experiment period,the results were as follows:Grass feeding quantity increased significantly from 4.65±0.231 to 6.413±0.039 kg/d(P≤0.001).The concentration of insulin-like growth factor-1(IGF-1)in serum increased significantly from 153.647±18.670 to 223.993±19.450 ng/ml(P≤0.01).The concentration of growth hormone(GH) in serum increased significantly from 23.170±0.891 to 26.792±1.003 ng/ml(P≤0.01).The concentration of insulin in serum increased significantly from 18.103±1.035 to 23.983±1.600 ng/ml(P≤0.01).The concentration of amino nitrogen in serum increased significantly from 0.102±0.003 to 0.140±0.004 mM/ml(P≤0.01).The concentration of ammonia nitrogen in serum decreased significantly from 0.206±0.012 to 0.086±0.006 mM/ml(P<0.001).Both the abomasum fluid of escape protein from rumen and common rumen fluid could enhance myocardium contraction force significantly,and the effect was reversed significantly by naloxone,so it could prove that the different digesta had the activity of exorphins.All those proved that using protein by-passs rumen protection technique plant exorphins effectively enhanced regulation effects in growth metabolism and utilization ratio of forage protein was increased as well.

关 键 词：蛋白质 过瘤胃保护 植物 外啡肽 山羊 生长代谢 调节作用 

分 类 号：S827[农业科学—畜牧学]