水通道蛋白亚型AQP1-9在雄性小鼠生殖系统mRNA的表达  

AQP1-9 mRNA expression of water channel in reproductive tract of male mouse

作　　者：赵丹[1] 杜建时[2] 杨柯[1] 杨宝学[3] 赵雪俭[1] 

机构地区：[1]吉林大学基础医学院病理生理学教研室,吉林长春130021 [2]吉林大学中日联谊医院血管外科,吉林长春130031 [3]美国加利福尼亚大学旧金山医学院,美国旧金山ca94122

出　　处：《吉林大学学报（医学版）》2004年第1期52-55,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅资助课题 (吉科合字第 2 0 0 1 1 1 0号 )

摘　　要：目的 :明确 AQP1 - 9在雄性小鼠生殖系统的分布与表达。方法 :应用 RT- PCR技术检测 AQP1 - 9在雄性小鼠生殖系统不同器官的表达。结果 :在成年雄性小鼠的生殖系统中 ,有多种水通道的表达 ,睾丸中有 AQP3、5、 7、 8、 9表达 ;附睾头中有 AQP3、 6、 8表达 ;附睾尾有 AQP3、 5、 6、 7、 8表达 ;输精管中有 AQP1、 2、 3、4、 5、 6、 7的表达。结论 :小鼠生殖系统中多种水通道蛋白的表达提示Objective\ To determine the expression and distribution of aquaporin 1-9(AQP1-9)in male reproductive tract of mouse. Methods The expressions of AQP1-9 in various organs of male reproductive tract of mouse was determined using RT PCR. Results The expression of AQP3,5,7,8,9 were present in mouse testis. AQP3,6,8 expression were found in capita epididymis and AQP3,5,6,7,8 in caudae epididymis. In vas deferens, the AQPs except for AQP8,9 were detected. Conclusions The different localization of AQP1-9 expression in male reproductive system of mouse shows that they may play an important role in physiological function of male reproductive system,especially for the maturation of sperm in different segments of male reproductive tract of mouse.

关 键 词：水孔蛋白/代谢 睾丸/代谢 附睾/代谢 输精管/代谢 疾病模型 动物 

分 类 号：R339.21[医药卫生—人体生理学] R-332[医药卫生—基础医学]