高温诱导Hep-2细胞凋亡及其对细胞周期进程的影响  

Study on mechanism of Hep-2 cell apoptosis induced by high temperature and its effect on cell cycle progress

作　　者：冯青杰[1] 杜柏山[2] 舒畅 李刚[4] 孙立群[1] 

机构地区：[1]吉林大学第二医院耳鼻咽喉科,吉林长春130041 [2]吉林大学第二医院麻醉科,吉林长春130041 [3]黑龙江省大庆市龙南医院 [4]吉林省磐石市医院

出　　处：《吉林大学学报（医学版）》2004年第1期103-104,共2页Journal of Jilin University：Medicine Edition

摘　　要：目的 :通过高温诱导 Hep- 2细胞凋亡探讨细胞凋亡和细胞周期的关系。方法 :应用 MTT比色法和流式细胞仪检测高温诱导 Hep- 2细胞凋亡和细胞周期时相的改变。结果 :高温组与对照组之间 Hep- 2细胞凋亡率差异有显著性 (P<0 .0 1 )。 MTT比色法高温组与对照组之间 ,Hep- 2细胞增殖抑制率差异亦有显著性。结论 :高温可提高 Hep- 2细胞的凋亡率 ,抑制 Hep- 2细胞的增殖 ,抑制 Hep- 2细胞 G1 期向Objective To study the relationship between apoptosis and cell cycle with Hep 2 cell apoptosis induced by high temperature.Methods To examine Hep 2 cell proliferation inhibitory rate,apoptosis and different phase of cell cycle with MTT colorimetry and flow cytometry.Results The difference of Hep 2 apoptosis rate between heating group and control group was obviously significant;The different of Hep 2 proliferation inhibitory rate between heating group and control group with MTT colorimetry was obviously significant ( P <0 01).Conclusion High temperature can increase Hep 2 cell apoptosis and inhibit the cell cycle progress of Hep 2 from G 1 stage to S stage.

关 键 词：细胞凋亡/生理学 细胞周期/生理学 高温 诱发 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] Q68[医药卫生—基础医学]