热激处理与水稻雄性不育系育性转变关系的初步研究  

A PRELIMILARY STUDY ON THE RELATIONSHIP BETWEEN HEAT SHOCK RESPONSE AND POLLEN FERTILITY TRANSFORMATION OF A CYTOPLASMIC MALE STERILE LINE OF RICE(ORYZA STIVA)

作　　者：许仁林[1] 师素云[1] 易琼华 

机构地区：[1]江苏省农业科学院遗传生理研究所,南京210014

出　　处：《武汉植物学研究》1992年第4期297-304,共8页Journal of Wuhan Botanical Research

基　　金：农业部"七.五"重点课题

摘　　要：本实验对水稻雄性不育系珍汕97A及其保持系在幼苗(2叶或3叶)和孕穗期(抽穗前8天)分别进行一次热激处理,并比较了其同工酶组成、花粉育性以及自交结实性的变化。同工酶分析结果表明,热激处理能使不育系与保持系花药的POX和Est的表达发生不同的改变,导致两系在这两种同工酶组成上的原有差异缩小;育性观察结果显示,在正常温度条件下,热激处理对珍汕97A的育性表达几乎无影响,而在有高温作用(穗分化发育阶段的日平均温度高于30℃)的条件下,3叶期幼苗往后的热激处理似乎能不同程度地提高珍汕97A的育性稳定性。简要讨论了热激处理与不育系育性转变间的关系。In this experiment heat shock to the CMS line Zhenshan 97A and its maintainer line were performed in seedlings with 2 or 3 leaves or plants at booting stage (8 days before earing). The changes of isoenzyme patterns, pollen constitutions and self-fertility have been detected. The results obtained by electrophoretic analysis showed that heat shock treatment could induce different expression of peroxidase and esterase isozyme in anther tissue, consequently, lead to reducing the distinction of the constitutions of these two isoenzymes between CMS line and its maintainer. On the other hand, observations indicated that heat shock treatment had little effect on the expression of the CMS phenotype if the head differentiation stage of Zhenshan 97A was under normal atmospheric condition. Heat shock treatment to seedlings with 3 leaves and afterwards developing stage, however, might enhance the stability of the CMS character when the head differentiation stage of the CMS line encountered high temperature(daily mean temperature was higher than 30℃). The relationship between the response to heat shock and the pollen fertility transformation induced by high temperature of the CMS line was briefly discussed.

关 键 词：水稻 育性转变 热激 雄性不育 

分 类 号：S511.101[农业科学—作物学]