Smad3基因剔除对小鼠肾脏功能的影响  

Influence on Kidney Function in Smad3 Gene Knock-out Mice

作　　者：王冬平[1] 孙岩松[1] 杨晓[2] 李文龙[1] 栾蓉晖[1] 战大伟[1] 李吉庆[1] 时彦胜[1] 李桂军[1] 张爱兰[1] 

机构地区：[1]军事医学科学院实验动物中心,北京100071 [2]军事医学科学院生物工程研究所,北京100071

出　　处：《中国比较医学杂志》2003年第4期227-230,共4页Chinese Journal of Comparative Medicine

基　　金：北京市科技项目专项基金 ( 95 49810 0 0 )

摘　　要：目的　了解Smad3基因剔除对小鼠肾脏解剖特征及功能的影响。方法　常规解剖学方法及通过荷兰半自动生化仪检测有关一些肾脏功能的血清学指标。结果　不同日龄不同基因型Smad3基因剔除小鼠之间的比较 ,35日龄Cre、体重、肾脏、肾上腺差异显著 ,UA、BUN纯合型小鼠高于野生型 ,体重及脏器重量与脏器指数低于野生型 ;70日龄和 6月龄BUN、体重、肾脏重量差异显著 ,6月龄纯合型小鼠的Cre和BUN高于野生型 ,70日龄纯合型小鼠的UA高于野生型 ,而 6月龄的UA却低于野生型。纯合型小鼠在不同年龄时各项指标差异不显著 ,但Tbili、Cre、BUN在 6月龄时比较高 ,UA在 70日龄相对高。结论　Smad3基因剔除小鼠从性成熟经过体成熟到老龄阶段 ,其代谢产物存在差异 ;通过解剖学观察Smad3基因剔除小鼠 ,含有纯合基因的小鼠在生产繁殖过程 ,出现 12 5 %的单肾小鼠 ,而肾上腺尚在。说明Smad3基因剔除对小鼠肾脏的形成具有一定影响。Objective To study the anatomic feature and the function of kidney in Smad3 gene knock-out mice. Methods The blood parameters of kidney drainage were measured by anatomical method and by VITALAB. Results There were significant difference in mice between types and age of Smad3 gene knock-out mice the homozygous mutants . The parameters of Cre,avoirdupois, kidneys and adrenal gland were significant differences between three types in 35 days mice :the parameters of the UA and BUN were higer than those of heterozygous mutants ,and the parameters of avoirdupois and visceral coefficient were lower than those of wildtypes ,but the parameters of Cre and BUN were higher than wildtypes. The parameters of UA of 70 days homozygous mutants were higher than wildtypes, but UA of 6 months lower. There were no significant differences in homozygous mice ,but parameters of Tbili ,Cre and BUN were higher in 6 months, the UA was higher in 70 days. Conclusion The metabolite outcomes were different in different ages of the mice . There were 12.5% one-kidney-mice which were produced by homozygous mutants,but the adrenal glands in existence. It was showed that Smad3 gene konck-out affected the formation of the kidneys.

关 键 词：SMAD3基因 小鼠 肾脏功能 生物化学 血清学 

分 类 号：R692[医药卫生—泌尿科学] R-332[医药卫生—外科学]