FK506对大鼠脑液压伤后血浆一氧化氮合酶、一氧化氮、内皮素和脑组织兴奋性氨基酸水平的影响  

The Effect of FK506 on Plasm Levels of Nitricoxide Synthase, Nitrogen Monoxide,Endothelins and Tissue Excitatory Amino Acids Following Brain Fluid Percussion Injury in Rats

作　　者：李秋平[1] 张桂运[1] 崔尧元[1] 高慧[2] 

机构地区：[1]复旦大学附属中山医院神经外科,上海200032 [2]复旦大学医学神经生物国家重点实验室,上海200032

出　　处：《中国临床医学》2003年第4期495-497,共3页Chinese Journal of Clinical Medicine

摘　　要：目的 :分析创伤性脑损伤 (TBI)后FK5 0 6对血浆一氧化氮合酶 (NOS)、一氧化氮 (NO)、内皮素 (ET)和脑组织兴奋性氨基酸 (EAA)水平的影响。方法 :雄性SD大鼠采用液压损伤法制备脑损伤模型 ,随机分为损伤组、治疗组和对照组。治疗组于伤后 5min开始每 6h腹腔注射 1次FK5 0 6 ,连续 4次。伤后 30min、6h、2 4h测量血浆NO、NOS、ET和伤灶脑组织的EAA水平。结果 :与对照组相比 ,治疗组NOS活性与NO ,EAA水平显著降低 ,ET水平几乎没有变化。结论 :按照本实验设计 ,FK5 0 6能抑制伤后NO升高 ,减少EAA释放 ,对TBI后继发性脑损伤有较大应用价值。Objective: To investigate the effect of FK506 on plasm levels of nitricoxide synthase(NOS), nitrogen monoxide(NO), endothelins(ET) and tissue excitatory amino acids(EAAs) following fluid percussion injury in rat. Methods: The male Sprague-Dawley rats were used as the TBI models made by fluid percussion device. The models were randomly divideded into groups of injury, treatment and control. FK506(1mg/kg body weight) was administered to the group of treatment four-times by intraperitoneal injection every six hours following TBI. The rats were killed respectively for testing the levels of NOS, NO, ET and tissue EAAs at the time of 30 minutes, 6 hours and 24 hours after TBI. Results: Compared with the group of injury the activity NOS and the levels of NO, EAAs descended significantly and the level of ET almost unchanged in the group of treatment. Conclusion: According to the present design FK506 can refrain NO from ascending and reduce the release of EAAs.

关 键 词：FK506 大鼠 创伤性脑损伤 血浆 一氧化氮合酶 一氧化氮 内皮素 兴奋性氨基酸 

分 类 号：R651.15[医药卫生—外科学]