Cytological and Molecular Identification of Alien Chromatin in Giant Spike Wheat Germplasm  

巨穗小麦种质中外源遗传物质的细胞遗传学和分子生物学鉴定(英文)

作　　者：窦全文[1,2] 陈佩度[1] 解俊峰[3] 

机构地区：[1]南京农业大学农业部作物细胞遗传重点实验室 [2]中国科学院西北高原生物研究所,西宁810008 [3]中国科学院西北高原生物研究所

出　　处：《Acta Botanica Sinica》2003年第9期1109-1115,共7页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金(39893350)。~~

摘　　要：Alien chromosomes of twelve giant spike wheat germplasm lines were identified by C-banding, genomic in situ hybridization (GISH), sequence characterized amplified region (SCAR), and random amplified polymorphic DNA (RAPD). All lines showed a chromosome number of 2n = 42, five of them carried both a pair of wheat-rye (Triticum aestivum-Secale cereal) 1BL/1RS translocation chromosomes and a pair of Agropyron intermedium (Ai) chromosomes, three carried a pair of Ai chromosomes only, three others carried a pair of 1BL/1RS chromosomes only, and one carried neither 1BL/1BS nor Ai chromosome. Further identification revealed that the identical Ai chromosome in these germplasm lines substituted the chromosome 2D of common wheat (T aestivum L.), designated as 2Ai. The genetic implication and further utilization of 2Ai in wheat improvement were also discussed.利用C分带、基因组原位杂交并结合分子生物学手段,对12份巨穗小麦种质材料中的外源遗传物质进行了检测。结果表明,12份材料染色体数均为42,其中5份材料均具有一对小麦-黑麦(Triticum aestivum-Secalecereal)1BL/1RS易位染色体和一对中间偃麦草(Agropyron intermedium Garten)染色体、3份材料只具有一对中间偃麦草染色体、3份材料只具一对1BL/1RS染色体、1份材料无1BL/1RS和中间偃麦草染色体。进一步细胞学分析表明,此中间偃麦草染色体代换了普通小麦(Triticum aestivum L.)中的2D染色体,因其良好的同源补偿性,表示为2Ai。同时对2Ai在巨穗小麦种质中存在的遗传学意义及小麦遗传改良中的应用进行了讨论。

关 键 词：giant spike germplasm 1 BL/1 RS Agropyron intermedium C-banding genomic in situ hybridization (GISH) sequence characterized amplified region (SCAR) random amplified polymorphic DNA (RAPD) 

分 类 号：S512.101[农业科学—作物学]