Expression of a Wheat S-like RNase (WRN1) cDNA During Natural-and Dark-induced Senescence  

小麦类核糖核酸酶基因(WRN1)cDNA在自然衰老和黑暗诱导衰老条件下的表达(英文)

作　　者：常胜合[1] 英加[1] 张吉军[1] 苏俊英[1] 曾雅娟[1] 童依平[2] 李滨[1] 李振声[1] 

机构地区：[1]中国科学院遗传与发育生物学研究所,北京100101 [2]中国科学院生态环境研究中心,北京100101

出　　处：《Acta Botanica Sinica》2003年第9期1071-1075,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家重点基础研究发展规划项目(G1998010200)。~~

摘　　要：An S-like RNase cDNA had been isolated from common wheat (Triticum aestivum L). The transcription of WRN1 mRNA was down-regulated by natural- and dark-induced senescence. But it was not senile-tissue-specific. As the two key histidine residues were replaced, WRN1 may not be active as RNase. Southern blotting analysis showed that WRN1 exists as one of a small gene family in common wheat genome.从普通小麦(Triticum aestivum L.)中分离了一个类核糖核酸酶(WRN1)基因的cDNA。WRN1的转录受自然衰老和黑暗诱导衰老的负调控。在幼嫩组织中WRN1也有表达。由于在两个保守的位置上组氨酸被替换,WRN1很可能已经失去了核糖核酸酶的活性。 Southern分析表明,在普通小麦基因组中,WRN1以一个小基因家族的形式存在。

关 键 词：wheat S-like RNase (WRN1) leaf senescence common wheat 

分 类 号：S512.101[农业科学—作物学]