一氧化氮预防腹膜粘连的实验研究  

Role of nitric oxide in prevention of postoperative adhesion formation in mice

作　　者：刘付宝[1] 刘生光[1] 何长林[1] 刘瑞林[1] 

机构地区：[1]蚌埠医学院附属医院普外科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2004年第1期5-8,共4页Journal of Bengbu Medical College

摘　　要：目的 :探讨一氧化氮 (NO)在小鼠实验性腹膜创伤后腹膜粘连形成过程中的作用。方法 :昆明小鼠 80只 ,预实验组 8只小鼠采用改良Mazuji方法制作实验性手术创伤模型 ,检验该方法致腹膜粘连之效果。实验组 72只小鼠随机分为Ⅰ、Ⅱ、Ⅲ组 ,按上述方法制作实验性手术创伤 ,分别于关腹前、术后连续 3天腹腔内注入生理盐水液 (Ⅰ )、L 精氨酸生理盐水液 (Ⅱ )和NG 硝基精氨酸甲酯生理盐水液 (Ⅲ )。术后 3(a)、7(b)、1 4 (c)天各组分别随机取 8只小鼠自眼球后采血测定血清NO值 ;采用Nair分级标准记录粘连得分和切取粘连肠管组织测定羟脯氨酸 (OHP)值两项指标来评价粘连严重程度。结果 :预实验组 :术后 2周处死小鼠 ,粘连发生率 1 0 0 % ,均为中、重度粘连 (3～ 4级 ) ,平均粘连得分 3.5 0± 0 .5 3。实验组 (1 )粘连严重程度 :术后第 3天各组间粘连得分及组织OHP值很低且几乎相等 (P >0 .0 5 ) ,第 7、1 4天Ⅰ、Ⅲ组明显高于Ⅱ组 (P <0 .0 1 ) ;a亚组平均粘连得分及OHP值最低 ,c亚组最高 ,b亚组其次 (P <0 .0 1 )。且在b、c亚组平均粘连得分与OHP值有明显相关性 (P <0 .0 1 )。 (2 )血清NO值 :Ⅱ组明显高于Ⅰ、Ⅲ组 (P <0 .0 1 ) ,且Ⅰ、Ⅲ组之间差异无显著性 (P >0 .0 5 ) ;组间比较a、b、c亚组NO值差异均无显?Objective:To investigate the effect of nitric oxide in preventing postoperative adhesions formation in traumatic mice.Methods:In the pre experimental group eight kunming mice were created cecal abrasion and parietal peritoneal defect with vefined Mazuji methods as experimental models and the effect of adhesion formation was observed.In the experimental group,seventy two mice were divided into control group(Ⅰ),L arginine group(Ⅱ) and NG nitroarginine methyl ester(L NAME,Ⅲ) group.0.9% NaCl(Ⅰ),L arginine(Ⅱ) and L NAME(Ⅲ) were administered intraperitoneally before abdominal closure and 3 consecutive days after surgery.All the three groups were divided into three subgroups for evaluation of adhesion severity and serum nitric oxide(NO) levels at postoperative days 3,7 and 14.Adhesion severity was measured with adhesion grading(Nairs's standard)and tissue hydroxyproline(OHP) levels.Results:In the pre experimental group,the adhesion rate was 100% in the eight mice two weeks after operation.All adhesions were middle or severe adhesion(3 to 4 grade) and the mean score was 3.50±0.53.In the experimental group,(1)the adhesion mean score and OHP levels were minimal and almost equal between the three groups at day 3(subgroup A)( P >0.05),while the adhesion mean score and OHP levels were higher in groupⅠand Ⅲ than in group Ⅱ at day 7(subgroup B) and 14(subgroup C)( P < 0.01 ).The adhesion mean score and OHP levels were the highest in subgroup A,the lowest in subgroup C and the medium in subgroup B( P <0.01).Logistic regression analysis showed significant correlation between adhesion score and tissue OHP levels in subgroup B and C respectively( r \-b=0.95, r \-c=0.93, P <0.01).(2)NO level was higher in group Ⅱ than in groupⅠand group Ⅲ at day 3,7 and 14 respectively( P <0.01)and there was no difference between groupⅠand group Ⅲ(P>0.05).There was no difference between subgroup A,B and C( P >0.05).Conclusions:The intraperitoneal administration of L arginine results in signif

关 键 词：一氧化氮 粘连 创伤 腹膜 

分 类 号：O613.61[理学—无机化学] R572.2[理学—化学]