贝那普利减轻肾小球硬化机制及与肾脏细胞凋亡关系的研究  

The expression of glomerulosclerosis by benazepril and its relationship with apoptosis of kidney cells

作　　者：韩子明[1] 王宏伟[1] 邢燕[2] 梁秀玲[3] 周建华[1] 

机构地区：[1]华中科技大学同济医学院,湖北武汉430030 [2]新乡医学院,河南新乡453003 [3]泰山医学院附属医院,山东泰安271000

出　　处：《实用儿科临床杂志》2004年第1期15-17,共3页Journal of Applied Clinical Pediatrics

基　　金：河南省教委资助项目 (1 99932 0 0 2 3)

摘　　要：目的　探讨血管紧张素转化酶抑制剂 (ACEI)减轻肾小球硬化的机制。方法　单侧肾切除大鼠加阿霉素注射诱导肾硬化模型 ,治疗组每日予贝那普利 6mg/kg灌胃一次 ,共 12周。采用TUNEL法检测肾脏细胞凋亡 ,免疫组化法检测肾组织Fas和FasL表达 ,病理分析软件进行量化分析。结果　模型组较对照组肾小球硬化明显 ,肾实质凋亡细胞数显著增多 ,Fas和FasL表达亦明显增强 ;贝那普利治疗组肾小球硬化较模型组明显减轻 ,肾细胞凋亡数显著较少 ,Fas和FasL表达亦减弱。结论　ACEI可能通过降低凋亡相关蛋白Fas及FasL在肾组织表达而抑制肾脏细胞过度凋亡 。Objective To study the effects of angiotensin converting enzyme inhibitor benazepri1 on apoptosis and the expression of Fas and FasL in the kidney of rats with adriamycin-indued nephritic glomeruosclerosis.Methods After uninephrectomy and the injection of adriamycin induced rats model with glomerulosclerosis, benazapril(6 mg/kg) was delivered daily by gavage to the rats in therapeutic groups for 12 weeks.Apoptosis was examined by means of terminal-deoxynucleotidyl trans ferase mediated d-UTP nick end label ling(TUNEL) and immunohistochemistry was utlized to detect the expression of Fas and FasL.Software of pathological analysis quantitated the level of Fas and FasL.Results Compared with those of the control group, the kidney of model group had moresevere glomerulosclerosis, much more apoptotic cells and higher level of exprssion of Fas and FasL. The degree of glomeruloscleroais, the nuxner of apoptotic cells and the level of expression of Fas and FasL were ameliofated by benazepril treatment.Conclusion Benazepril may suppress the excessive apoptosis of kidney cell by lowering the expression of the protin correlatng apoptosis Fas and FasL,so as to postpone the process of glomeruosclerosis.

关 键 词：血管紧张素转化酶抑制剂 贝那普利 阿霉素肾病 细胞凋亡 Fas FASL 

分 类 号：R965[医药卫生—药理学]