温度和盐度对球形棕囊藻细胞DMSP产量的影响  

EFFECTS OF TEMPERATURE AND SALINITY ON DMSP PRODUCTION IN PHAEOCYSTIS GLOBOSA

作　　者：王艳[1] 齐雨藻[1] 沈萍萍[1] 李韶山[2] 吕颂辉[3] 

机构地区：[1]暨南大学生命科学和技术学院,广州510632 [2]华南师范大学生命科学学院,广州510631 [3]1暨南大学生命科学和技术学院,广州510632

出　　处：《水生生物学报》2003年第4期367-371,共5页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金 (批准号 :3 0 2 0 0 0 3 0 );国家重点基础研究发展规划 ( 973 )项目 (编号 :001CB409704);广东省"十五"科技重大专项 (编号:2002A3050103 ) ;广东省教育厅环保科技项目 (粤高教科 [1998] 75号 );广州市科委重点攻关计划项目 (计划编号 :98

摘　　要：球形棕囊藻汕头株 (Shantoustrain ,ST)和香港株 (Hongkong ,HK)是DMSP与DMS的高产株 ,在 2 0℃、4 0‰盐度的培养条件下 ,二者DMSP产量分别达到 15 6 1 35和 4 37 6 0nmol/10 6cells。细胞内DMSP的积累与释放到细胞外DMS量受盐度、温度等环境因子的影响 :在高盐低温条件下 ,单位藻细胞的DMSP与DMS产量较高。香港株DMSP/DMS的积累和释放与生长时期有关 ,稳定期细胞内的DMSP含量高达 3898 5 3nmol/10 6cells,是指数期的 12 3倍。The algal osmolyte dimethylsulfoniopropionate(DMSP)is recognized as the major precursor of marine dimethylsulfide(DMS),a volatile sulfur compound that affects atmospheric chemistry and global climate. In method of GC-MS,effects of temperature and salinity on DMSP production in both Shantou and Hong Kong strains of Phaeocystis globosa were investigated in the present study. DMSP production in Shantou strain at 20℃ and salinity 40‰ was 1561.35nmol/10 6cells,but in Hong Kong strain under the same culture condition was only 437.60nmol/10 6cells which was about one third to one fourth that in Hong Kong strain. Intracellular DMSP accumulation and release of DMS were greatly affected by temperature and salinity. DMSP/DMS productivity in both strains was increased by high salinities and low temperatures. P. globosa at different growth phases showed different DMSP/DMS productivity. The content of the intracellular DMSP in the stable phase was much higher than that exponential phase. In stable phase of Hong Kong strain,DMSP content was 3898.53 nmol/10 6cells,which was 12.3 times of that in exponential phase. Intraspecific differences in DMSP production in P. globosa under laboratory conditions were observed in the present study. The results indicated that both Shantou and Hong Kong strains of P. globosa were HAB(harmful algal bloom)species with high DMSP production. It was suggested that DMSP may play additional important roles in biogeochemistry of the surface ocean when P. globosa red tide occurred in the South China Sea.

关 键 词：球形棕囊藻 温度 盐度 二甲基硫丙酸 二甲基硫化物 DMSP 产量 

分 类 号：Q949.25[生物学—植物学]