转化生长因子β_1和血小板衍化生长因子的表达与嗜酸粒细胞性支气管炎气道重建的关系  

The relationship between transforming growth factor-β_1, platelet derived growth factor and airway remodeling in eosinophilic bronchitis

作　　者：李怀臣[1] 张紧[2] 黄琛[1] 王茂芬[1] 姜淑娟[1] 牟晓燕[1] 林殿杰[1] 迟翔宇[3] 苏莉莉[1] 朱玲[1] 张才擎[1] 

机构地区：[1]山东省立医院呼吸内科,济南250021 [2]山东省立医院病理科,济南250021 [3]山东省立医院老年呼吸科,济南250021

出　　处：《中华结核和呼吸杂志》2003年第12期761-764,共4页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：目的　研究嗜酸粒细胞性支气管炎 (EB)气道管壁结构改变与转化生长因子 β1(TGF β1)和血小板衍化生长因子 (PDGF)在气道黏膜中表达之间的关系。方法　选择 12例EB(A组 )、10例哮喘 (B组 )和 10例早期周围型肺癌 (C组 )患者 ,经纤维支气管镜作肺下叶段支气管黏膜活组织检查 (简称活检 ) ,C组患者活检取健侧肺。苏木精 伊红 (HE)染色观察气道上皮下基膜 (SBM )厚度 ;免疫组化技术检测TGF β1和PDGF在气道黏膜中的表达。结果　A组SBM厚度为 (6 3± 1 4 ) μm、B组为 (8 2± 1 5 ) μm、C组为 (4 1± 1 2 ) μm ,三组比较差异有显著性 (P <0 0 5 ) ;A组气道黏膜以及黏膜下层中TGF β1和PDGF每平方毫米阳性细胞数分别为 (5 9± 9)个、(4 7± 7)个、B组分别为(85± 12 )个、(76± 11)个、C组分别为 (31± 4 )个、(2 0± 3)个 ,三组比较差异有显著性 (P <0 0 5 ) ,且TGF β1和PDGF与SBM厚度呈正相关关系 (r分别 =0 76、0 5 2 ,P <0 0 5 )。结论　EB患者可以出现类似于支气管哮喘的气道SBM增厚的病理改变 ,且TGF β1和PDGF在EB气道黏膜下纤维化的病理改变中发挥了重要作用。Objective To study the relationship between the expression of transforming growth factor-β 1(TGF-β 1) and platelet derived growth factor(PDGF) and airway remodeling in eosinophilic bronchitis(EB). Methods Bronchial biopsy specimens were obtained from 12 patients with EB(A group), 10 asthmatic patients(B group) and 10 patients(C group) with peripheral lung cancer in early stage. The subepithelial basement membrane(SBM) thickness was measured by light microscopy using HE staining. The expressions of TGF-β 1 and PDGF in the bronchial mucosa were examined by immunostaining. Results The SBM of A group[(6.3±1.4)μm] was significantly thicker than that of C group[(4.1±1.2) μm, [WTBX]P<0.05], but significantly thinner than that of B group, but significantly thinner than that of B group[(8.2±1.5) μm]. The numbers of positive cells for TGF-β 1 and PDGF in A group (59±9, 47±7 respectively) and B group (85±12, 76±11, respectively) were significantly higher than those in C group (31±4, 20±3, respectively), and were positively correlated with SBM thickness (r=0.76, 0.52, P<0.05). Conclusion These results suggest that TGF-β 1 and PDGF expressions in bronchial mucosa may play a role in bronchial subepithelial fibrosis in EB patients.

关 键 词：转化生长因子Β1 血小板衍化生长因子 嗜酸粒细胞性支气管炎 气道重建 气道黏膜 

分 类 号：R562.21[医药卫生—呼吸系统]