丹参酮ⅡA对肥厚心肌细胞核因子-κB的影响  

The effects of sodium tanshinone ⅡA sulfonate on activation of NF-κB on hypertrophic myocardial cells

作　　者：孙联平[1] 郑智[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院急诊科,武汉430030

出　　处：《实用老年医学》2004年第1期25-27,共3页Practical Geriatrics

基　　金：湖北省自然科学基金资助项目 ( 2 0 0 0J0 6 4)

摘　　要：目的　探讨丹参酮ⅡA对血管紧张素Ⅱ (AngⅡ )诱导的心肌细胞肥大的影响。　 方法　利用体外细胞培养技术 ,用AngⅡ刺激新生大鼠心肌细胞肥大 ,通过免疫组织化学方法观察丹参酮ⅡA对肥大心肌细胞核因子 (NF κB)表达的影响。　 结果　与正常细胞相比 ,AngⅡ组细胞直径明显增加 ,细胞核NF κB呈强表达。加入丹参酮ⅡA干预后 ,心肌细胞直径明显减小 ,NF κB表达减弱 (P <0 0 1)。　 结论　丹参酮ⅡA具有保护心肌 ,防止心肌肥厚作用 ,其抑制心肌细胞肥厚的机制可能与抑制NFObjective To explore the effects of sodium tanshinone ⅡA sulfona te on hypertrophy induced by angiotensin Ⅱ(Ang Ⅱ) in cultured neonatal rat myo cardial cells. Methods An in vitro cell culture technique was used. The effects of sodium tanshinone ⅡA sulfonate on expression of nuclear transcription factor κB (NF-κB) were studied by immunohistochemistry on angiotensin Ⅱ-induced hypertrophic myocytes. Results Compared with normal cultured myocytes, cardiom yocyte diameter in Ang Ⅱ group were higher and NF-κB expression increased sig n ificantly (P<0.01). Sodium tanshinone ⅡA sulfonate significantly suppresse d angiotensin Ⅱ-induced hypertrophy of cells and reduced NF-κB expression ( P<0.05). Conclusions Sodium tanshinone ⅡA sulfonate may be beneficial in p rotecting the myocardium against hypertrophy. The suppression of myocardial hype rtrophy might be associated with the suppression of NF-κB expression caused by sodium tanshinone ⅡA sulfonate.

关 键 词：丹参酮ⅡA 核因子-ΚB 血管紧张素Ⅱ 大鼠 

分 类 号：R972[医药卫生—药品] R364.31[医药卫生—药学]