Epstein -Barr病毒与肝炎病毒在肝细胞癌发生中的作用(英文)  

Role of Epstein-Barr Virus and Hepatitis Viruses in hepatocellular carcinogenesis

作　　者：李威[1] 林莹[2] 杜海军[3] 吴宝安[1] 李曼红[2] 曾毅[3] 曾永明[1] 陈君填[1] 郭毓文[1] 

机构地区：[1]汕头大学医学院第一附属医院普外一区,广东汕头515041 [2]汕头大学医学院第一附属医院病理科,广东汕头515041 [3]中国疾病预防控制中心病毒病预防控制所肿瘤室,北京100052

出　　处：《中国现代医学杂志》2004年第2期7-12,共6页China Journal of Modern Medicine

基　　金："TheroleofHepatitisvirusesandEBVinthecarcinogenesisofliverandbileductcarcinoma"isanitemofTechnologyprogramofGuangdongProvince(ItemNumber:2KM0 45 0 4s)

摘　　要：目的　EBV作为肿瘤相关病毒已获公认 ,为探讨EBV与HCC的关系及与肝炎病毒有无协同作用 ,进行本研究。方法　采用PCP、RT -PCR及免疫组化方法检测石蜡包埋HCC标本中EBV、HBV、HCV和HDV。结果　PCR测HBVDNA(X基因和 (或 )S基因 )阳性率为 5 6 .4 % (4 4例 /78例 ) ,EBVDNA(BaimHⅠW和 (或 )LMP1)阳性率为 2 8.2 % (2 2例 /78例 )。RT -PCR测HCVRNA阳性率为 5 .6 % (1例 /18例 ) ,HDVRNA阳性率为 0 (0例 /18例 )。免疫组化测EBVLMPl多定位于肿瘤细胞中。结论　EBV在HCC组织中有较高的检出率 ,值得注意 ;HBV对HCC发生起重要作用 ,与EBV无明显关系 ;本地区HCV发病率不高 ,但其与EBV在HCC发生中有无协同作用值得进一步研究。HDV与HCC无明显关系。Objective: It's well known that EBV is tumor-associated virus. The aim of this study was to find out the infection state of EBV in Hepatocellular Carcinoma (HCC) patients, and discuss whether EBV acts as a helper virus and cooperates with hepatitis viruses in HCC carcinogenesis.Methods:Polymerase Chain Reaction (PCR) and Reverse Transcription PCR (RT-PCR) were processed to detect EBV (BamHI W and (or) LMP1), HBV (X gene and/or S gene), HCV and HDV in paraffin sections from HCC patients. Immunohistochemistry method was used to identify the character and location of gene expression in 22 cases whose EBV DNA were positive.Results: EBV DNA was detected in 28.2%(22 in 78) of the HCC tissues by PCR while the positive rate of HBV DNA was 56.4%(44 in 78). HCV RNA was detected in 5.6%(1 in 18) of HCC tissues by RT-PCR while the result of HDV RNA was negative (0 in 18). Positive signals of LMP1 were mainly localized in the tumor cells while the positive ratio of HCV was 13.6%(3 in 22)by immunohistochemistry method.Conclusions: The existence of EBV infection in HCC tissues suggests that EBV may have some relations with HCC. HBV infection is a major cause of HCC but EBV and HBV have no obvious correlation in the development of HCC. Whether EBV DNA detected by PCR method is from carcinoma cell or interstitial lymphocyte?More experiments must be done. The beforehand experiments show that perhaps there is correlation between EBV and HCV in the development of HCC, so the research of this correlation will be the focus of next phase research. There is no direct role for HDV in HCC carcinogenesis.

关 键 词：肝肿瘤/病因学 疱疹病毒4型 人 肝炎病毒 

分 类 号：Q939.4[生物学—微生物学]