凝结芽胞杆菌产碱性脂肪酶的条件研究  

Studies on alkaline lipase producing conditions by bacillus coagulans

作　　者：吕飒音[1] 胡磊 

机构地区：[1]安徽大学现代实验技术中心,安徽合肥230039 [2]安徽省休宁啤酒厂,安徽休宁245400

出　　处：《安徽大学学报（自然科学版）》2004年第1期69-72,共4页Journal of Anhui University(Natural Science Edition)

摘　　要：本文对分离获得的产碱性脂肪酶的5株菌进行复筛,对产酶量大的一株菌F12进行鉴定,确定该菌株为凝结芽胞杆菌(BacillusCoagulans)。此菌产碱性脂肪酶的最适条件是:黄豆饼粉2.5,玉米浆1.5,可溶性淀粉0.5,K2HP40.5,NaNO30.5,起始pH9.0,培养温度28～30℃,培养周期22～26h。A bacterium producing alkaline lipase was re-screened from five strains. The alkaline lipase activity was the highest in strain F12 and studies revealed that this strain is Bacillus coagulants. The optimal conditions of the bacterium strain producing alkaline lipase is (%): soy bean cake flour 2.5, corn steep liquor 1.5, soluble starch 0.5, K2HPO4 0.5, NaNO3 0.5, initial pH9.0, culture temperature 28℃～30℃ for 22～26 hour.

关 键 词：凝结芽胞杆菌 产碱性脂肪酶 微生物 油脂 

分 类 号：Q939.124[生物学—微生物学]