A群链球菌制剂抗肿瘤效应与细胞因子的关系  

The Relation between the Antineoplastic Effect of Group A Streptococcus and Cytokines

作　　者：曾刚[1] 徐培君[2] 邹志宏[1] 

机构地区：[1]南京医科大学附属苏州医院呼吸科,215000 [2]江苏省苏州医学院病原生物学教研室,215000

出　　处：《医药导报》2004年第2期77-79,共3页Herald of Medicine

摘　　要：目的 :从细胞因子水平探讨A群链球菌制剂的抗肿瘤机制。方法 :将浓度 0 .5,0 .1,0 .0 2 ,0 .0 0 4KE·mL 1的A群链球菌制剂与肿瘤患者外周血淋巴细胞 (PBL)共同孵育 ,以活性细胞定量 (MTT)法检测经 2 4,48,72h诱导后的PBL对K562及Raji细胞的杀伤效应 ,并采用酶联免疫吸附 (ELISA)法或生物学活性检测法测定其上清液中白细胞介素 12 (IL 12 )、白细胞介素 2 (IL 2 )、γ 干扰素 (IFN γ)、α 肿瘤坏死因子 (TNF α)含量。结果 :经不同浓度A群链球菌制剂作用不同时间后PBL对K562及Raji细胞的杀伤效应不同 ,诱导产生的细胞肿瘤因子也不同。 0 .1和 0 .0 2KE·mL 1A群链球菌制剂可增强PBL杀伤肿瘤细胞的能力 ,其上清液中亦均能检测到较高水平的IL 12、IL 2、IFN γ、TNF α;0 .5KE·mL 1A群链球菌制剂不但抑制PBL对肿瘤细胞的杀伤 ,而且还抑制其分泌细胞因子的能力。结论 :A群链球菌制剂诱导肿瘤患者PBL杀伤肿瘤细胞的活性与其浓度有关 。Objective:To probe into the mechanisms of th e antineoplastic effect of group A streptococcus preparation sapeilin at the cytok ine level. Method:Sapeilin in concentrations of 0.5, 0.1, 0.02 and 0.004 KE·mL -1 was co-cultured with peripheral blood lymphocytes (PBL) form tumor patients. After 24, 48 and 72 hours of co-culture, respectively the PBL were subjected to assay of their cytotoxicity directed against K562 cells an d Raji cells with the MTT method. Meanwhile detection of IL-12, IL-2, IFN-γ and TNF-α in supernatants of the PBL was carried out either with FLISA or meas urement of bioactivity. Results:The cytotoxic effects of and the cytouines produ ced by the PBL which had been co-cultured with sapeilin in different concentrat ions and for different periods of time were shown to be different. Sapeilin in c oncentrations of 0.1 and 0.02 KE·mL -1 was shown to enhance the cytotoxic effect of PBL directed against tumor cells and high levels of IL-12, IL-2, IF N-γ and TNF-α could also be detected in the supernants. Sapeilin in a concen tration of 0.5 KE·mL -1 was shown not only to depress the cytotoxic effec t of PBL directed against tumor cells but also decrease the amounts of cytokines produced by the PBL. Conclusion:The cytotoxic effect of PBL from tumor patien ts directed against tumor cells was shown to be related to the concentration of sapeilin co-incubated with the PBL. The tumoricidal effect was also shown to be related to the production of cytokines.

关 键 词：A群链球菌 抗肿瘤效应 细胞因子 

分 类 号：R977.3[医药卫生—药品]