菟丝子中槲皮素药代动力学研究  

Pharmacokinetic Studies of Quercetin in Semen Cuscutae

作　　者：曾诚[1] 宓穗卿[2] 罗颂平[1] 黄天来[2] 洪馨[2] 骆运晖[1] 

机构地区：[1]广州中医药大学第一附属医院,广州510405 [2]广州中医药大学临床药理研究所,广州510405

出　　处：《中药新药与临床药理》2004年第1期37-40,共4页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的建立菟丝子中槲皮素的含量测定方法,研究槲皮素体内动力学规律。方法建立快速敏感的高效液相色谱-紫外检测法(HPLC-UV),以卡马西平为内标,以菟丝子灌胃给药后,大鼠含药血清样品经液-液萃取后在C18反相色谱柱上进行分离测定。结果槲皮素血药浓度在0.05～9μg/mL浓度范围内线性关系良好(r=0.9998)。方法回收率为86.65～99.07%,提取回收率为79.91～84.56%,最低检测限为0.05μg/mL。结论槲皮素在大鼠体内的动力学过程表现为具吸收的二室开放模型,其中t1/2(Ab)=0.13h,t1/2(α)=0.19h,t1/2(β)=1.22h,tmax=0.333h。实验结果可为菟丝子在临床用药的安全性和有效性提供依据。Objective To establish the method of measuring quercetin content in semen cuscutae(SC) and to explore its in- vivo pharmacokinetics features.Methods The rapid and sensitive HPLC- UV method was adopted with carbamazepine acting as internal standard. After the extract of SC was given, serum samples of rats were collected and measured on C18 reverse- phase chromatographic column after liquid- liquid extract. Results The linear range of calibration curve were 0.05～ 9μ g/mL (r=0.9998). The method showed good precision and recoveries for serum with 86.65～ 99.07% , and extracting recoveries 79.91～ 84.56% .The limit of detection(LOD) as 0.05μ g/mL. Conclusion The pharmacokinetic process of quercetin in vivo manifestated an opening two- compartment model with t1/2(Ab)=0.13h, t1/2(α )=0.19h, t1/2(β ) =1.22h, tmax=0.333h. The results may provide evidence of safety and effectiveness for clinical use of SC.

关 键 词：菟丝子 槲皮素 卡马西平 高效液相色谱法 药代动力学 

分 类 号：R285.5[医药卫生—中药学]