Broad host range plasmid-based gene transfer system in the cyanobacterium Gloeobacter violaceus which lacks thylakoids  

作　　者：GUOHaitao XUXudong 

机构地区：[1]InstituteofHydrobiology,ChineseAcademyofSciences,Wuhan430072,China

出　　处：《Progress in Natural Science:Materials International》2004年第1期31-35,共5页自然科学进展·国际材料（英文版）

基　　金：SupportedbytheOne HundredTalentsProjectoftheChineseAcademyofSciences

摘　　要：Gloeobacter violaceus , a cyanobacterium lack of thylakoids, is refractory to genetic manipulations because its cells are enveloped by a thick gelatinous sheath and in colonial form. In this study, a large number of single cells were obtained by repeated pumping with a syringe with the gelatinous sheath removed. And an exogenous broad host range plasmid pKT210 was conjugatively transferred into G. violaceus. Analyses with dot blot hybridization and restriction mapping showed that the exogenous plasmid pKT210 had been introduced into G. violaceus and stably maintained with no alteration in its structure. pKT210 extracted from G. violaceus exconjugants could be transformed into the mcr- mrr- E. coli strain DH10B but not the mcr+ mrr+ strain DH5α, which suggests that a methylase system may be present in G. violaceus.Gloeobacter violaceus , a cyanobacterium lack of thylakoids, is refractory to genetic manipulations because its cells are enveloped by a thick gelatinous sheath and in colonial form. In this study, a large number of single cells were obtained by repeated pumping with a syringe with the gelatinous sheath removed. And an exogenous broad host range plasmid pKT210 was conjugatively transferred into G. violaceus. Analyses with dot blot hybridization and restriction mapping showed that the exogenous plasmid pKT210 had been introduced into G. violaceus and stably maintained with no alteration in its structure. pKT210 extracted from G. violaceus exconjugants could be transformed into the mcr- mrr- E. coli strain DH10B but not the mcr+ mrr+ strain DH5α, which suggests that a methylase system may be present in G. violaceus.

关 键 词：Gloeobacter violaceus CONJUGATION pKT210 

分 类 号：Q933[生物学—微生物学] Q782