间充质干细胞与人发角蛋白人工腱联合培养的形态学  

Morphology of human mesenchymal stem cells and HHK artificial tendon co-culture

作　　者：付文玉[1] 路艳蒙[2] 朴英杰[2] 安连兵[2] 乔东访[2] 

机构地区：[1]潍坊医学院组织学与胚胎学教研室,潍坊261042 [2]第一军医大学中心实验室

出　　处：《解剖学杂志》2003年第4期312-315,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金重点项目(39830390);国家重点基础研究发展规划(973)(G1999054308);国家自然科学基金项目(30070368);广州市科委科技攻关项目(2000-Z-017-01-05)

摘　　要：目的:研究培养的人骨髓间充质干细胞(MSCs)与人发角蛋白(HHK)人工腱的相容性以及MSCs在HHK上的生长情况。方法:人MSCs与HHK人工腱联合培养后,分别在倒置显微镜及扫描电镜和透射电镜下观察MSCs的生长状态和超微结构。结果:MSCs在光镜下呈单层或多层贴附于HHK人工腱表面,扫描电镜下呈细长梭形,有的呈扁平多角形,其生长方向与HHK人工腱长轴相一致。透射电镜下可见静息态和活动态两种不同功能状态。结论:MSCs与HHK人工腱之间有极好的粘附性和相容性,是一种理想的组织工程种子细胞。Objective : To investigate the compatibility between human marrow-derived mesenchymal stem cells (MSCs) and human hair keratin (HHK) artificial tendon in vitro. Methods : The co-cultured human MSCs and HHK artificial tendon were studied using inverted microscope and scanning electron microscope; the ultrastructure of MSCs was observed under transmission electron microscope. Results : Under inverted microscope, the single or multi-layer MSCs adhered to the surface of each hair of HHK artificial tendon. Most MSCs growing on the surface of HHK artificial tendon had long spindle; some were flat and multi-angle in shape. Static MSCs and active MSCs could be identified under transmission microscope. Conclusion : MSCs have good compatibility with HHK artificial tendon, and the MSCs are ideal seed cells for tissue engineering.

关 键 词：间充质干细胞 人发角蛋白 人工腱 联合培养 形态学 骨髓 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]