新型纳米银-磷酸锆-中性红复合膜修饰电极对过氧化氢电催化还原  

The Electrocatalytic Reduction of Hydrogen Peroxide by the Novel Ag_n^0-Zirconium Phosphate-Neutral Red Complex Film Modified Electrode

作　　者：张国荣[1] 王艳玲[1] 

机构地区：[1]山东师范大学化学系,济南250014

出　　处：《分析化学》2003年第12期1421-1424,共4页Chinese Journal of Analytical Chemistry

摘　　要：制备了新型纳米银 磷酸锆复合材料 ,对中性红有强烈吸附。吸附的中性红表现出更强的氧化还原性 ,氧化还原中点电位与溶液中 (pH 7)相比正移约 2 0 0mV。将辣根过氧化酶与复合材料共同修饰在电极表面制成酶电极 ,对H2 O2 有显著电催化还原作用。催化电流在 2 .5× 1 0 - 6 ～ 2 .0× 1 0 - 3mol L范围内与H2 O2 浓度成正比 ;H2 O2 检出限为 2 .0× 1 0 - 7mol L(S N =3 ) ;传感器对 1 .0× 1 0 - 5mol LH2 O2 响应时间 <1 5s;RSD为1 4% (n=1 0 )。4℃保存 6个月该电极仍有 85 %以上初始响应。传感器有效消除了抗坏血酸等共存物质的干扰 ,回收率实验结果令人满意。The novel composite material consisting of nanosize silver and zirconium phosphate (ZP) was prepared and modified on the surface of electrodes after it adsorbed neutral red (NR). The neutral red retained in the film exhibits an enhanced redox reaction ability. The midpoint potential ( E _m) of adsorbed NR was 200mV more positive than that of NR in the solution of pH 7. The H_2O_2 biosensor was constructed with the film made from the Ag 0_n-ZP colloid with horseradish peroxide (HRP), bovine serum albumin and glutaraldehyde. This sensor has the significant effect of electrocatalytic effect on the reduction of H_2O_2. Its catalytic current was linear with the concentration of H_2O_2 in the range of 2.5×10 -6 ～2×10 -3 mol/L. The detection limit of this sensor was 2×10 -7 mol/L H_2O_2( S/N 3). The 95% steady-state response time was less than 15 s. More than 85% of the initial response was retained after the storage for 6 months at 4℃. Particularly the sensor effectively eliminated the interference from most of coexistent substances such as ascorbic acid. The results of the recovery rate experiments were satisfactory.

关 键 词：纳米银-磷酸锆-中性红复合膜修饰电极 过氧化氢 电催化还原 生物传感器 辣根过氧化酶 

分 类 号：O657.1[理学—分析化学]