毛细管电泳前沿分析法研究钾离子与18-冠醚-6体系相互作用  

Investigation of Interaction of Potassium Ion to 18-Crown-6 by Capillary Electrophoresis/Frontal Analysis

作　　者：刘勇[1] 刘玉明[1] 夏之宁[2] 

机构地区：[1]四川大学分析测试中心,成都610064 [2]重庆大学化学化工学院,重庆400044

出　　处：《分析化学》2003年第12期1500-1503,共4页Chinese Journal of Analytical Chemistry

摘　　要：采用毛细管电泳前沿分析 (CE FA)法 ,利用间接紫外检测方法 ,在pH为 5 .1 0 ,运行电压为 3 0kV ,缓冲溶液组成为咪唑和醋酸的条件下 ,测定了钾离子与 1 8 冠醚 6体系的相互作用参数 ,结合常数对数值lgK =3 .5 0 ;在同样条件下用峰漂移法作对照实验 ,求得体系的结合常数对数值lgK =3 .40 ,结果与前沿分析法基本一致。验证了两种方法测定结果的正确性。A capillary electrophoresis/frontal analysis (CE/FA) method has been applied to study the interaction parameter of potassium ion with 18-crown-6. The binding constant, which is IgK = 3.50, was obtained with indirect ultraviolet detection in the imidazole and acetate buffer solution (pH = 5.10) at 30 running voltage. Furthermore, the electrophoretic peak shifting model has also been used to determine the binding constant of the system under the same condition. The result is IgK = 3.40, which coincides with the datum measured by the CE/FA.

关 键 词：钾离子 18-冠醚-6体系 相互作用 毛细管电泳 前沿分析 结合常数对数值 生物膜 

分 类 号：O658.9[理学—分析化学]