小型可连续进样微流控芯片分析仪的研制  

The Development of a Compact Microfluidic Chip Analyzer with Continuous Sample Introduction

作　　者：徐章润[1] 王世立[1] 樊晓峰[1] 王福仁[1] 方肇伦[1] 

机构地区：[1]东北大学分析科学研究中心,沈阳110004

出　　处：《分析化学》2003年第12期1527-1530,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目 (No .2 9870 0 1)

摘　　要：报道了一种结构简单、可连续进样的小型微流控芯片分析仪的研制。顺序注射分析系统通过芯片上制作的接口将试样连续引入芯片 ,并采用自行设计的紧凑型光纤式激光诱导荧光检测器进行检测。该仪器用于芯片毛细管电泳分离实验室合成Cy5荧光染料 ,实现了连续进样和换样。峰高RSD为 1 .9% (n=1 1 ) ,试样通量 3 5 h ;相邻试样携出 <4%。A novel microfluidic chip analyzer was developed, in which the capillary electrophoresis (CE) separation with laser induced fluorescence (LIF) detection, simplified optics involving the use of optical fibers, capable of non-interrupted continuous sample introduction using a sequential injection (SI) system were used. The performance of the system is demonstrated in the separation of fluorochrome Cy5 from impurities. Low carryover (<4%) among samples was achieved at a sampling frequency of 35/h with a precision of 1.9% RSD ( n 11).

关 键 词：小型可连续进样微流控芯片分析仪 微流控分析系统 光纤式激光诱导荧光检测器 顺序注射分析技术 

分 类 号：TH89[机械工程—仪器科学与技术]