环境激素DBP对拟南芥试管形态发生的影响  

Effects of Di-butyl Phthalate on morphogenesis of Arabidopsis thaliana in vitro

作　　者：王晓娟[1] 金樑[2] 陈家宽[2] 

机构地区：[1]同济大学现代农业科学与工程研究院设施农业技术研究所,上海200092 [2]复旦大学生命学院生物多样性研究所生物多样性与生态工程教育部重点实验室,上海200433

出　　处：《西北植物学报》2003年第11期1889-1893,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：上海市科技兴农攻关项目(农科攻字2000第5-12号)

摘　　要：报道了酞酸酯类化合物DBP对拟南芥试管形态发生的影响。利用不同浓度的DBP处理拟南芥茎段外植体,切片观察拟南芥茎段试管形态发生过程中脱分化、分化及再分化的变化情况。结果发现:拟南芥茎段外植体在对照组培养基上生长良好,首先是愈伤组织产生,而后愈伤组织块增大,颜色呈淡绿色,生长旺盛,并有分化出芽现象;添加0.01mg/L、0.1mg/L和1.0mg/LDBP,茎段愈伤组织诱导率低,且高浓度时愈伤组织出现褐化、活力差的现象;培养12h、24h、2d、3d、7d、14d、21d和28d茎段显微切片结果显示,DBP具有抑制拟南芥试管形态发生的作用,随着DBP浓度的增加呈现剂量抑制效应。Di-butyl phthalate (DBP) is one of the common environmental contaminants found both in terrestrial and aquatic ecosystems.However,little is known about its effects on plant morphogenesis.We report here a study of examining the DBPs effects on morphogenesis of Arabidopsis thaliana.When stem segments of Arabidopsis thaliana were cultured on MS medium supplemented with 2 mg/L 6-BA and 0.2 mg/L NAA,callus could be induced and some shoots could be regenerated.However,the presence of DBP with different concentrations (0.01 mg/L,0.1 mg/L,1.0 mg/L) in the above medium inhibited callus formation of stems,and the presence of high concentration of DBP (1.0 mg/L) eventually led to the death of the calluses.Microphotographs of longitudinal sections of stem segments cultured for 12 h,24 h,2 d,3 d,7 d,14 d,21 d and 28 d showed that DBP inhibited the dedifferentiation and differentiation of Arabidopsis stem explants;and the effect was more obvious along with the increase of DBP concentration in the medium.

关 键 词：拟南芥菜 DBP 分化 脱分化 形态发生 

分 类 号：Q944.6[生物学—植物学]