九州虫草菌丝体多糖Ck_1-A的纯化及其性质研究  

PURIFICATION AND PROPERTES OF POLYSACCHARIDE Ck_1-A FROM CULTURED MYCELIA OF CORDYCEPS KYUSHUENSIS

作　　者：王延兵[1] 吕鹏[1] 张长铠[1] 

机构地区：[1]山东大学微生物技术国家重点实验室,济南250100

出　　处：《菌物系统》2003年第3期452-456,共5页Mycosystema

基　　金：教育部访问学者基金资助。

摘　　要：九州虫草Cordyceps kyushuensis 菌丝体粗多糖经酶法结合Sevag法除蛋白、H2O2法脱色、透析、乙醇分部沉淀得到Ck1,Ck1经DE-23柱层析得到多糖Ck1-A。经紫外扫描、聚丙烯酰胺凝胶电泳、Sepharose 4B柱层析等方法检测确定Ck1-A为均一组分。苯酚-硫酸法测得Ck1-A的含糖量为86.1%,硫酸-咔唑法测得Ck1-A含有微量的葡萄糖醛酸。Sepharose 4B柱层析测得Ck1-A的分子量为927 kDa。红外光谱和核磁共振氢谱的结果均显示Ck1-A含有α-型糖苷键。体内实验表明Ck1-A对小鼠肉瘤S180有明显的抑制作用。Ck1 was obtained from crude polysaccharide of Cordyceps kyushuensis by removing proteins using Sevag method ,decolourization using H2O2 method, dialysis and alcohol-fractional. A polysaccharide Ck1-A was isolated from Ck1 by DE-23 chromatography .Its homogeneity was verified by UV scan, polyacrylamide gel electrophoresis and gel-filtration chromatography. Its carbohydrate content was 86.1% .It contains a little quantity of uronic acid . The average molecular weight of Ck1-A was 927000 Da. The results of IR and `H-NMR show that Ck1-A has a α-glycoside linkage. Ck1-A was found to have marked effects on Sarcoma 180 in vivo .

关 键 词：离子交换层析 结构测定 抑瘤 

分 类 号：R284[医药卫生—中药学]