用RT—PCR扩增人谷胱甘肽过氧化物酶的cDNA  

AMPLIFYING cDNA OF GLUTATHIONE PEROXIDASE BY RT-RCR

作　　者：李广元[1] 闫小君[2] 任瑛云 杜晓阳[1] 李信民[1] 

机构地区：[1]西安医科大学中西医结合研究所,西安710061 [2]第四军医大学生物化学教研室

出　　处：《西安医科大学学报》1992年第3期203-205,共3页Journal of Xi'an Medical University(Chinese)

摘　　要：本文应用反转录-聚合酶链反应成功的扩增了人谷胱甘肽过氧化物酶的互补脱氧核糖核酸(cDNA)。该法首先从人肝癌细胞中提取信使核糖核酸,在反转录酶的作用下生成cDNA,以此CDNA作为PCR的模板,利用人工合成的两个特异引物,进行35次循环的扩增。电泳结果表明,扩增片段的大小约为600 bp,与文献结果一致,为该酶基因的表达及生产奠定了基础。The glutathione peroxidase cDNA has been acquired by reverse transcriplate-polymerase chain reaction (RT-PCR). mRNA was isolated from the culture liver cancer cells firstly, then trancriplate if into cDNA with AMV reverse transcriptase. By using this cDNA as PCR template and two synthetic special primers, we get about 600 bp DNA fragment after 35 circles. The result is identical with our expectation.

关 键 词：谷胱甘肽 过氧化物酶 克山病 GPX 

分 类 号：R542.302[医药卫生—心血管疾病]