氧自由基对人胚软骨细胞DNA基质成分及超微结构的影响  

THE EFFECT OF OXYGEN FREE RADICAL ON DNA ,MATRIX CMPONENT SYNTHESIS AND ULTRASTRUCTUREOF HUMAN EMBRYO CHONDROCYTES

作　　者：朱杰[1] 魏西秦[1] 张健[1] 朱宝琳[1] 

机构地区：[1]西安医科大学病理解剖教研室,西安710061

出　　处：《西安医科大学学报》1992年第4期325-328,共4页Journal of Xi'an Medical University(Chinese)

摘　　要：本实验采用体外软骨细胞培养方法,观察由黄嘌呤—黄嘌呤氧化酶产生的自由基对人胚软骨细胞DNA、基质蛋白多糖、胶原合成功能及超微结构的影响。结果表明该酶系产生的自由基抑制人胚软骨细胞DNA、蛋白多糖、胶原的合成,对软骨细胞膜及细胞器具有明显的损伤作用,从而间接说明某些炎性关节疾病时活化中性粒细胞、巨噬细胞释放的氧自由基是引起关节软骨损伤的重要因素。By using the method of chondrocytes in monolayer culture in vitro we observed the effect of free radicals generated by the xanthine oxidase and xanthine on DNA, matrix proteoglycan (PG), collagen synthesis and ultrastructure of human embryo chondrocytes. Our results showed that the synthesis of DNA, PG and collagen of the chondrocytes were obviously inhibited and the membranes and subcellular organelles of the chondrocytes were damaged strongly. On the bases of these results we suggest that oxygen free radicals from poymorphonuclear leukocytes and phagocytes would be the important factors of cartilage injury in arthritis.

关 键 词：软骨细胞 自由基 关节炎 

分 类 号：R684.302[医药卫生—骨科学]