苏云金芽孢杆菌的特异性结合蛋白研究  

Study on Relation of Cry1A and Cry1C Gene Promoter Upstream with Specific Binding Proteins

作　　者：程萍[1] Arthur I.Aronson 喻子牛[3] 

机构地区：[1]珠海市农业科学研究中心,广东珠海519070 [2]美国Purdue大学生物系,Laffeyete,Indiana,USA [3]华中农业大学微生物科学技术系农业部农业微生物重点实验室,湖北武汉430070

出　　处：《微生物学杂志》2004年第1期14-18,共5页Journal of Microbiology

基　　金：美国国家科学基金 (NationalSciencefoundationofUSA)的资助 (MCB -960 0 5 84)

摘　　要：用3 2 P分别标记 3 0 8bpcry1A上游和 65 0bpcry1C上游片段 ,并将标记后的DNA与不同苏云金芽孢杆菌菌株的细胞粗蛋白进行凝胶阻滞反应。结果表明 ,cry1A和cry1C上游均能被苏云金芽孢杆菌库斯塔克亚种 (Bacillusthuringinensis subsp .kurstaki)的细胞粗蛋白特异性结合 ,而同一cry1基因上游序列可被不同多肽特异或非特异性竞争结合 ,不同的cry1基因上游序列也能同时被一种蛋白结合。说明苏云金芽孢杆菌某些特异细胞蛋白参与了cry1基因上游序列的转录调控作用 ,而不同的调节因子可能会竞争同一结合位点。库斯塔克亚种和鲇泽亚种 (B .thuringinensis subsp .aizawai)所含特异细胞蛋白在种类和作用上都有差异。In the study,+{32}P was used directerly for marking 308bp of cry1A upstream sequences and 650bp of cry1C upstream sequence. Then gel retardation was done between the marked DNA sequences and the cell full proteins of different strains of Bacillus thuringiensis. The results shown that the upstream of cry1A and cry1C could be retarded by the crude proteins in the cells of subsp.kustaki. And a upstream of cry1 gene was retarded by different poly-peptides, specially by non-specially. At same time, the upstream sequences of different cry1 gene were also retarded by one kind of protein. It illuminated that some specific proteins joined the transcriptional regulation of cry1 gene upstream. And different regulators might compete same binding sites. The specific proteins of subsp. kurstaki and subsp.aizawai were different in kinds and functions.

关 键 词：苏云金芽孢杆菌 杀虫晶体蛋白基因 启动予上游序列 结合蛋白 凝胶阻滞反应 生物杀虫剂 

分 类 号：Q933[生物学—微生物学]