青藤碱对吗啡依赖离体豚鼠回肠作用的实验研究  

Effect of sinomenine on morphine dependence in isolated guinea pig ileum

作　　者：王彩云[1] 莫志贤[1] 涂宏海 

机构地区：[1]第一军医大学中医系药理药化教研室 [2]兰州军区68210医院

出　　处：《第一军医大学学报》2003年第4期329-331,共3页Journal of First Military Medical University

基　　金：国家自然科学基金(30271610)~~

摘　　要：目的探讨青藤碱对吗啡依赖性的戒断作用及机制。方法建立豚鼠离体回肠模型,以纳络酮催促吗啡依赖性戒断收缩,观察10、50和250 μmol/L Sin及0.1 μmol/L尼莫地平在加入纳络酮前后给药对戒断性收缩的影响;观察青藤碱对乙酰胆碱引起的正常豚鼠回肠收缩的作用。结果青藤碱及尼莫地平均可抑制纳络酮催促的戒断性收缩,对乙酰胆碱引起的正常豚鼠回肠收缩也有抑制作用,青藤碱的作用呈剂量依赖趋势。结论青藤碱能够抑制吗啡依赖的戒断症状。Objective To investigate the effects of sinomenine on morphine withdrawal response and acetylchline (Ach)-in-duced contracture in isolated guinea pig ileum. Methods The withdrawal contracture was elicited by subjecting isolated ileum incubated with morphine (3 μmol/L) at 37.5 ℃for 4 h to naloxone (1 μmol/L) treatment. Sinomenine (10, 50, 250 μmol/L) and nimodipine (Nim, 0.1 μmol/L) were administered 1 min before and after naloxone in morphine-dependent ilea bathed in Krebs solution containing morphine, to observe the changes in the withdrawal contracture of the ileum. The effect of sinomenine (10, 50, 250 μmol/L) on the contracture of untreated ileum in Krebs solution elicited by acetylcholine was also observed. Results and Conclusion Naloxone-induced withdrawal contracture or acetylcholine-induced contracture of the ileum was significant-ly decreased in a dose-dependent manner, indicating that sinomenine can inhibit morphine withdrawal symptoms in guinea pigs.

关 键 词：吗啡依赖 青藤碱 戒断作用 作用机制 豚鼠离体回肠模型 

分 类 号：R285.5[医药卫生—中药学]