铜绿微囊藻抗性株与野生株对铜离子的富积效应  

Bioaccumulation of copper ion in resistant and wild strains of Microcystis aeruginosa

作　　者：刘红涛 侯桂琴[2] 席宇[3] 赵以军[4] 薛乐勋[2] 

机构地区：[1]郑州大学细胞生物学研究室 [2]郑州大学细胞生物学研究室郑州450052 [3]郑州大学生物工程系 [4]华中师范大学生命科学学院

出　　处：《郑州大学学报（医学版）》2004年第1期54-57,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目 3 9970 0 64 ;教育部优秀青年教师基金 教人司 ( 2 0 0 1) 3 9号资助项目

摘　　要：目的 :分离筛选高铜离子抗性的铜绿微囊藻 (Microcystisaeruginosa) ,并研究其抗性机制及对铜离子的富积效果。方法 :将抗性株与野生株分别加入不同浓度铜离子的BG1 1培养基中 ,在不同时间段取样过滤 ,分析过滤液中铜离子的残留量。结果 :在相同时间内 ,抗性株比野生株产生类金属硫蛋白 (MTL)的量要大 ,培养基中Cu2 + 减少主要发生在培养后 2 4h以前。抗性株在 2 4h之前对Cu2 + 富积较快 ,2 4h的去除率为 4 8.87% ,96h的去除率为80 .77% ;而野生株在 2 4h之前对Cu2 + 富积相对较慢 ,2 4h去除率为 2 0 .88% ,96h的去除率为 34.2 9%。结论Aim: A strain of Microcystis aeruginosa with high tolerance of copper ion was isolated, and its resistant mechanism and bioaccumulation were preliminarily studied. Methods: The resistant and wild strains were added to the BG11 cultures of different concentrations of Cu 2+ , the M.aeruginosa was filtrated and the contents of residual Cu 2+ in the filtrate were analyzed. Results: The resistant strain of M. aeruginosa could produce more metallothionein-like (MTL) than the wild strain under the same conditions. The decrease of Cu 2+ in BG11 medium mainly took place 24 h after the culture. The resistant strain of M. aeruginosa accumulated the maximal Cu 2+ about 24 h after the culture. The Cu 2+ removal rates of the resistant strain at 24 h and 96 h were 48.87% and 80.77%, and the biodegradation rates of the wild strain at 24 h and 96 h were 20.88% and 34.29%, respectively. Conclusion: Treating contaminated areas by Cu 2+ using the resistant strain of M. aeruginosa may have good application future.

关 键 词：铜绿微囊藻 抗性株 野生株 CU^2+ 富积 

分 类 号：Q949.220.5[生物学—植物学]