铜离子对铜绿微囊藻生长及生理的影响  

Effect of copper ion on the growth and physiology of Microcystis aeruginosa

作　　者：刘红涛 李杰 席宇[2] 赵以军[3] 薛乐勋[1] 

机构地区：[1]郑州大学细胞生物学研究室,郑州450052 [2]郑州大学生物工程系,郑州450052 [3]华中师范大学生命科学学院,武汉430079

出　　处：《郑州大学学报（医学版）》2004年第1期57-60,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目 3 9970 0 64 ;教育部优秀青年教师基金 教人司 ( 2 0 0 1) 3 9号资助项目

摘　　要：目的 :研究铜绿微囊藻在铜离子胁迫下的生长及生理学效应和其培养的最佳铜离子浓度。方法 :根据铜绿微囊藻在不同浓度铜离子中的生长 ,光合速率、超氧化物歧化酶 (SOD)活性及类金属硫蛋白 (MTL)的变化进行实验。结果 :①在 1 0 -5～ 1 0 -2 mol/L范围内 ,Cu2 + 浓度越高 ,对微囊藻生长的抑制越强 ,铜绿微囊藻生长最适的Cu2 + 的浓度为 1 0 -6mol/L。②低浓度 (1 0 -7～ 1 0 -6mol/L)的Cu2 + 对铜绿微囊藻SOD活性没有诱导作用 ,但可以作为微量元素促进铜绿微囊藻的生长 ;中浓度 (1 0 -5～ 1 0 -4mol/L)的Cu2 + 能够诱导铜绿微囊藻SOD活性增强 ;而高浓度 (1 0 -3 ～ 1 0 -2 mol/L)的Cu2 + 对铜绿微囊藻细胞产生毒性作用 ,抑制铜绿微囊藻生长。③ 1 0 -5mol/LCu2 + 能诱导铜绿微囊藻产生MTL ,且量最大 ;1 0 -6mol/LCu2 + 诱导铜绿微囊藻产生MTL的量较小 ;1 0 -3 mol/LCu2 + 对铜绿微囊藻生长有很强的抑制作用 ,诱导生成MTL的量最少。 6～ 9d诱导MTL的量最大 ,抗性株在相同时间内诱导MTL的量远大于野生株。结论 :不同浓度的铜离子可影响铜绿微囊藻的生长及生理活动 ,1 0 -6mol/L的铜离子是铜绿微囊藻生长的最适浓度。Aim: Growth and physiological activities of Microcystis aeruginosa were elucidated under Cu 2+ stress conditions. Additionally, the most suitable Cu 2+ concentration for the purified strain of M.aeruginosa was shown. Methods: The growth, photosynthetic rate, superoxide dismutase(SOD) activities and changing of metallothionein-like (MTL) of M.aeruginosa under the different concentration Cu 2+ were conducted. Results: 1.When solutions ranging from 10 -2 mol/L to 10 -5 mol/L of Cu 2+ , inhibitory effect appeared on the growth of M.aeruginosa; moreover, the greater the concentration of Cu 2+ , the more intensive inhibitory effect on the growth of M.aeruginosa. In contrast, Cu 2+ below 10 -5 mol/L was favorable to the growth of M.aeruginosa. The most favorable concentration of Cu 2+ for its growth was 10 -6 mol/L. 2. Cu 2+ at lower concentrations (10 -6 ～10 -7 mol/L) did not show inducible effect, but copper, as a trace element, had the stimulating effect on the growth of M. aeruginosa, Cu 2+ at mid-concentrations (10 -4 ～10 -5 mol/L) could induce SOD activities to rise, whereas Cu 2+ at higher concentrations (10 -2 ～10 -3 mol/L) poisoned the cells and displayed the inhibitory effect on the growth of M. aeruginosa. 3. Cu 2+ at 10 -5 mol/L induced M. aeruginosa to produce the maximum of MTL, but Cu 2+ at 10 -3 mol/L showed obviously inhibitory effect on the growth of M.aeruginosa. In addition, the yield of MTL reached the maximum during the culture period of 6～9 days and the resistant strain of M. aeruginosa could produce more MTL than the wild strain under the same conditions. Conclusion: Heavy metal copper could markedly affect the growth and physiological activities of M. aeruginosa, the results provide theoretical basis for further study of ecological action of M. aeruginosa.

关 键 词：铜绿微囊藻 铜离子 生长 生理 

分 类 号：Q949.220.5[生物学—植物学]