STAR蛋白QKI在去甲肾上腺素诱导大鼠心肌肥厚时的表达变化  

Expression of STAR protein QKI in norepinephrine-induced rat cardiac hypertrophy

作　　者：陈锴[1] 宋峣[1] 闫洁[1] 李平[1] 李劲梁[1] 张幼怡[1] 

机构地区：[1]北京大学第三医院血管医学研究所,分子心血管学教育部重点实验室,北京100083

出　　处：《中国病理生理杂志》2004年第1期1-5,共5页Chinese Journal of Pathophysiology

基　　金：国家重点基础研究发展规划项目(973)(G2 0 0 0 0 5690 6);国家自然科学基金资助项目(No.3 0 2 0 0 3 2 1)

摘　　要：目的 :探讨QKImRNA及蛋白在SD大鼠病理性心肌肥厚时的表达。方法 :采用去甲肾上腺素制备SD大鼠心肌肥厚模型 ,利用实时荧光定量PCR法和蛋白质免疫印迹法检测大鼠心肌肥厚时QKImRNA和蛋白质的表达变化。结果 :在SD大鼠心脏中有 3种QKI异构体mRNA和QKI蛋白的表达 ;当去甲肾上腺素持续输注引起大鼠心肌肥厚时 ,心脏QKI -5mRNA和QKI蛋白质表达水平均明显降低 (P <0 0 5) ;去甲肾上腺素诱导培养的乳鼠心肌细胞肥大时 ,QKI -5mRNA表达量也明显低于对照 (P <0 0 5)。结论 :大鼠心脏有QKImRNA和蛋白质表达 。AIM: To study the expression profile of QKI m RNA and protein in rat heart during pathological cardiac hypertrophy. ME THODS: A rat cardiac hypertrophy model was established using continuous norepinephrine (NE) perfusion. Real-time PCR and Western blotting were applied t o examine QKI mRNA and protein expression respectively in rat heart. RES ULTS: Both mRNA and protein of three QKI isoforms were detected in adult rat heart. QKI-5 mRNA and total QKI protein were remarkably decreased in NE-ind uced hypertrophic heart compared with those in control group (P<0 05). Furt hermore, the expression of QKI-5 mRNA also appeared to be lower than those in co ntrol group when the neonatal rat cardiomyocytes were incubated with NE. C ONCLUSION: Three QKI isoforms express in rat heart and they may particip ate in cardiac hypertrophy.

关 键 词：QKI蛋白质 去甲肾上腺素 心脏扩大 大鼠 心肌肥厚 STAR蛋白 表达 

分 类 号：R542.2[医药卫生—心血管疾病] R394[医药卫生—内科学]