α-平滑肌肌动蛋白和β-肌动蛋白在瘢痕组织中的表达  

Expression of α-SMA and β-actin in hypertrophic scar tissues

作　　者：马进[1] 范志宏[1] 

机构地区：[1]上海第二医科大学仁济医院整形外科,上海200127

出　　处：《上海第二医科大学学报》2004年第1期5-7,共3页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家自然科学基金资助项目(30000176)

摘　　要：目的 探讨α-平滑肌肌动蛋白(α-SMA)和β-肌动蛋白(β-actin)对增生性瘢痕形成的可能作用。方法 采用荧光定量PCR法检测10例增生性瘢痕和10例正常皮肤组织中α-SMA和β-actin的表达水平。结果 增生性瘢痕组织中α-SMA和β-actin表达水平均高于正常皮肤组织,差异有统计学意义(P<0.01)。结论 α-SMA和β-actin在瘢痕增生中起重要作用;由于β-actin在瘢痕组织和正常皮肤中的不恒定性,建议在瘢痕的mRNA定量研究中不作为内参照物。Objective To investigate the expressions of α-smooth muscial actin( α-SMA) and β-actin in hyper-trophic scar tissues and discuss their function in scar formation. Methods The expression levels of α-SMA and β-actin in specimens were determined by FQ-PCR technique. Results There were more expressions of α-SMA and β-actin in hypertrophic scar tissues than those in normal skin(P<0. 01) . Conclusion The expression differences of α-SMA and β-actin between the normal skin and hypertrophic scar tissues may contribute to the formation of scar. In scar mRNA quantitative research, β-actin is not suitable to be the reference standard because it fluctuates between the scar and normal skin.

关 键 词：Α-平滑肌肌动蛋白 Β-肌动蛋白 瘢痕组织 表达 

分 类 号：R622[医药卫生—整形外科]