人尿提取物抗肿瘤活性研究  

The antitumor activity of the extract from human urine

作　　者：麻亚锋[1] 陈钧辉[1] 张冬梅[1] 王新昌[1] 

机构地区：[1]南京大学生物化学系医药生物技术国家重点实验室,南京210093

出　　处：《中国药理学通报》2004年第1期69-71,共3页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助课题 NoBK970 3 6

摘　　要：目的　研究人尿提取物的抗肿瘤活性。方法　从人尿中提取到一种小肽混合物UAP(uricantitumorpeptides) ,用细胞计数法和MTT法测定UAP对 3种人肿瘤细胞SMMC772 1,MKN4 5和U937生长的抑制作用 ,并对其进行体内实验和急性毒性实验。结果　人尿中的提取物UAP对体外培养的SMMC772 1,MKN4 5和U937细胞具有抑制作用 ,但对人正常白细胞HNL(humannormalleucocyte)无明显抑制作用 ,体内实验表明UAP对小鼠HAC肝癌 ,小鼠LEWIS肺癌和小鼠S180 肉瘤瘤体生长有明显抑制作用。急性毒性试验显示其的毒性作用很低 ,LD50 =2 137 13mg·kg-1。AIM To study antitumor activity of the extract denominated as UAP(Uric Antitumor Peptides isolated and purified from normal human urine). MOTHODS Proliferation inhibition assay was examined by cell counting and MTT method. Experiments in vivo were tested by treatment on tumor mice and acute toxicity on healthy mice. RESULTS UAP exerted strong dose dependent inhibition on the proliferation of three tumor cell lines, but not human normal leucocytes. Treatments on tumor mice show that UAP effectively inhibited tumor growth of mice HAC hepatoma, mice Lewis lung carcinoma and mice S 180 sarcoma with apparently dose dependent effect. Acute toxicity proved that UAP had very low acute toxicity in mice with LD 50 = 2 137 13 mg·kg -1 . CONCLUSION UAP has strong antitumor activity in vitro and in vivo. This provides a promising alternative antitumor treatment.

关 键 词：人尿提取物 抗肿瘤活性 急性毒性 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R73-351[医药卫生—基础医学]