中国汉族及日本群体7个Y-STR位点及单倍型的遗传多态性和群体差异  

Genetic polymorphism and the population difference of 7 Y-STR loci and haplotypes in Chinese Han and J apanese populations

作　　者：赵东[1] 王保捷[1] 丁梅[1] 卢岩[1] 孙大成[1] 刘志芳[1] 李春梅[1] 

机构地区：[1]中国医科大学法医学院,辽宁沈阳110001

出　　处：《法医学杂志》2003年第3期143-148,共6页Journal of Forensic Medicine

基　　金：辽宁省科委科学基金资助项目(9810500601)

摘　　要：目的调查Y染色体7个STR位点及单倍型的遗传多态性并分析其群体差别。方法应用PCR、变性聚丙烯酰胺凝胶电泳结合银染显色分型技术,检测45例中国汉族及59例日本男性DNA样品。结果在DYS393、DYS389Ⅰ、DYS19、DYS390、DYS389Ⅱ、DYS392等6个位点中共检出33个等位基因,DYS385位点检出39个等位基因组,其频率分布在0.0169～0.6444之间,DP值分布在0.5406～0.9579之间,以DYS385位点最高。7个位点数据综合比较,二组群体间在遗传学上存在显著性差异P<0.05。由7个位点组成的单倍型有95种,中国汉族有41种,DP值为0.9960,日本群体有54种,DP值为0.9965,2群体间未发现相同的单倍型。结论上述7个STR位点属于高鉴别能力位点,单倍型具有很高的遗传多态性并显示出明显的民族特征。Objective To investigate the genetic polymorphism of7Y-STR loci and haplotypes and analyze their population difference.Methods The DNA samples from45Chinese Han and59Japanese men were ana-lyzed by PCR and the denatured PAGE followed by silver staining.Results33alleles were identified for6loci including DYS393,DYS389 Ⅰ ,DYS19,DYS390,DYS389 Ⅱ and DYS392,39alleles were identified for DYS385,the frequency distributions were between0.0169～0.6444,the DP value distributions of them were between0.5406～0.9579,the DP value of DYS385was the highest.With the comparison of data of the7loci,significant difference in genetics was observed between the two populations(P<0.05).There were95haplotypes that consist-ed of the7loci,Chinese Han had41haplotypes,DP value0.9960,Japanese population had54haplotypes,DP value0.9965,no common haplotypes were found between them.Conclusion The7STR loci above belong to loci of high discriminating ability,the haplotypes are highly polymorphic and their ethical character is apparent.

关 键 词：Y-STR 单倍型 遗传多态性 群体差异 个人识别 中国汉族 日本群体 

分 类 号：Q347[生物学—遗传学] D919.4[医药卫生—法医学]