离心力对制备沉积物间隙水中化合物浓度的影响  

EFFECT OF CENTRIFUGAL FORCE ON COMPOUNDS CONCENTRATIONS IN SEDIMENT INTERSTITIAL WATER

作　　者：朱江[1] 周永欣[2] 葛虹[3] 徐立铭[4] 张扬[3] 

机构地区：[1]中国地质大学工程学院,武汉430074 [2]中国科学院水生生物研究所,武汉430072 [3]湖北省水产科学研究所,武汉430071 [4]中国科学院武汉植物研究所,武汉430074

出　　处：《水生生物学报》2003年第5期487-491,共5页Acta Hydrobiologica Sinica

基　　金：农业部渔业环境基金资助 ;长江渔业资源管理委员会基金资助

摘　　要：研究了离心力对间隙水化合物浓度的影响。将腐殖质掺入东湖沉积物样品 ,按泥∶水 =1∶4 (体积 )比室温下静置 30d ,随后掺入铜 ,泥∶水 =1∶4 (体积 )比静置 10d ,得到总有机碳含量在 1 4 7%— 5 72 %、铜含量在 12 0— 2 70 0mg/kg(干重 )的试验沉积物样品 ,用离心法制备沉积物中的间隙水。离心参数为 30 0 0r/min、5 2 0 0r/min、90 0 0r/min、10 5 0 0r/min和 12 0 0 0r/min ,4℃下离心 2 0min。间隙水中化合物浓度分析表明 ,随离心力升高 ,间隙水中铜、铁、总有机碳 (TOC)含量逐渐降低 ,钙的含量略有增加 ,而锰的含量不受离心力变化的影响。根据上述分析结果 ,确定在腐殖质对沉积物中铜的毒性影响研究中 ,制备沉积物间隙水的适宜离心条件为 :12 0 0 0r/min、4℃下离心 2 0min。Freshwater sediment from Eastlake, Wuhan, China, was spiked with humus and copper to obtain a series of different levels of total organic carbon (TOC, 1.47%—5.72%)and total copper (120—2700mg/kg dry weight). In laboratory, humus was added to natural freshwater sediment and stored at 20℃ for 30 days in a volume portion of 1∶4 (sediment to water), then samples was spiked with copper and stored for 10 days. These humus and copper-spiked sediments were centrifuged under 3000r/min, 5200r/min, 9000r/min, 10500r/min, 12000r/min, 4℃ and 20min to collected its interstitial water (IW). chemica1 analysis showed that, Cu, Fe, TOC concentrations in IW decreased as centrifugal speed increased, meanwhile Ca somewhat increased but Mn concentration had no apparent correlation with centrifugal speed. According to the above results, the centrifugation method for interstitial water was preferable at: 12000r/min(9000g RCF), 4℃, 20min in study of humus effect on the toxicity of copper in sediment.

关 键 词：沉积物 间隙水 离心力 浓度 水生态系统 

分 类 号：Q178.1[生物学—水生生物学]