Tedenan保护兔膀胱出口部分梗阻后逼尿肌细胞的超微结构研究  

Studies of Protective Effect of Tedenan on Ultrastructure of Detrusor Cell Following Partial Outlet Obstruction in Rabbit Bladder

作　　者：王国民[1] 王杭[1] 钟慈声[2] 

机构地区：[1]复旦大学附属中山医院泌尿外科,上海200032 [2]上海医学院电子显微镜室,上海200032

出　　处：《复旦学报（医学版）》2003年第2期143-146,共4页Fudan University Journal of Medical Sciences

摘　　要：目的　探讨Tedenan(太得恩 )对兔膀胱出口部分梗阻后逼尿肌细胞超微结构的保护作用。方法　新西兰雄性白兔 2 0只 ,分 4组 ,每组 5只。建立兔膀胱出口部分梗阻动物模型后两周 ,太得恩治疗组给予太得恩每天 10 0mg/kg喂服 ,花生油组给予花生油 (太得恩赋形剂 ) ,梗阻组不给任何药物。饲养 3周后解剖膀胱 ,测定膀胱容量及重量 ,应用透射电镜分别放大 6× 10 3 倍观察内质网 ,放大 2× 10 4倍观察线粒体。结果　太得恩对兔膀胱出口部分梗阻所致的膀胱壁增生没有明显地抑制作用 ,而对膀胱容量缩小有明显的保护作用。太得恩组平均每个视野粗面内质网面积为 (1.2 5 9± 0 .2 74 ) μm2 ,对照组为 (0 .2 14± 0 .10 1) μm2 ,梗阻组为 (4 .75 0± 2 .15 2 ) μm2 ,花生油组为 (5 .76 1± 1.775 ) μm2 。单位视野内太得恩组内质网面积明显小于梗阻组及花生油组 (P <0 .0 5 ) ,但是明显大于对照组 (P <0 .0 5 )。太得恩组线粒体相对密度为 1.2 92± 0 .16 4 ,对照组为 1.5 82± 0 .2 86 ,梗阻组为0 .973± 0 .0 73,花生油组为 0 .92 5± 0 .10 2。对照组和太得恩组相对密度比较无明显差异 ,而明显高于梗阻组和花生油组 (P <0 .0 5 )。Purpose: To observe the protective effect of Tedenan on ultrastructure of detrusor cell in treating bladder dysfunction following outlet obstruction. Methods: Twenty New Zealand white male rabbits were divided into four groups of 5 rabbits each. Control group was received operation but not forming obstructive model; other groups were received operation forming obstructive model. Two weeks after operation, Tadenan group received Tadenan orally at 100 mg/(kg&middotd) for three weeks; peanut oil group received vehicle only(peanut oil) and the obstruction group did not receive any drugs for three weeks. Five weeks after operation, each rabbit was anesthetized and the bladder was rapidly removed and weighted. The ultrastructure including rough endoplactmic reticulum and mitochondrion of detrusor cell of each bladder were examined in electron-microscope. Results: (1) Tadenan did not reduce the bladder mass but had protective effect on the capacity of bladder following partial outlet obstruction. (2) The average area of rough endoplactmic reticulum in Tadenan group was (1. 259 ± 0. 274) μm2 in each unit cytoplasm area(114.68 μm2) versus (0. 214 ± 0. 101) μm2 in control group, (4. 750 ± 2. 152) μm2 in obstructive group, and (5. 761 ± 1. 775)μm2 in peanut oil group. (3) The relative density of mitochondrion was 1. 292 ± 0. 164 in Tedenan group versus 1. 582 ± 0. 286 in control group, 0. 973 ± 0. 073 in obstructive group and 0. 925 ± 0. 102 in peanut oil group. Conclusions: Tedenan have the protective effect on ultrastructure of detrusor cell. It is morphologic foundation of its protective effect on bladder function following partial outlet obstruction.

关 键 词：膀胱出口梗阻 逼尿肌细胞 超微结构 太得恩 药物治疗 

分 类 号：R694.2[医药卫生—泌尿科学] R983[医药卫生—外科学]