超声波骨组织电镜样品脱钙处理的实验研究  

An Experimental Study on Bone Decalcification in the Process of Sample Preparation for Electron Microscopy by Using Ultrasonic Irradiation

作　　者：法京[1] 沈强[1] 俞彰[1] 凌诒萍[1] 齐玲[1] 江家碗[1] 

机构地区：[1]复旦大学上海医学院电镜室,上海200032

出　　处：《复旦学报（医学版）》2003年第2期117-119,共3页Fudan University Journal of Medical Sciences

摘　　要：目的　探讨超声辐照能否加快骨组织电镜样品的脱钙过程。方法　固定后的样品浸入含 134mmol/L和NaOH 70mmol/L的脱钙液中 ,经 2 1kHz的超声波辐照 ,每天 2 0min共 7d。未经超声波处理的样品在脱钙液中浸泡 2 0d作为对照。在透射电镜下观察两组骨组织的超微结构。结果　经超声辐照的样品在骨基质中没有无机盐结晶残留 ,且超微结构保存良好 ,与对照组无差异。结论　超声辐照可使脱钙处理的时间大大缩短 。Purpose: To explore whether ultrasonic irradiation can shorten the process of osseous tissue decalcification during the sample preparation for electron microscopy. Methods: The fixed samples immersed in the decalcifying solution containing disodium ethylene diamino tetraacetic acid (EDTA&middotNa2) 134 mmol/L and Naoh 70 mmol/L were irradiated by ultrasonic waves at a frequency of 21 kHz for 20 min per day. The irradiation continued for 7 days. The samples immersed in the same decalcifying solution for 20 days without using the ultrasonic irradiation were used as the control. The ultrastructure of the osseous tissue in the two groups were observed under transmission electron microscope. Results: In the sections irradiated by the ultrasonic waves, there were no deposit of inorganic salt crystals shown on bone matrices, and the ultrastructure was preserved well. It seemed no difference from that of the control. Conclusions: Ultrasonic irradiation can shorten the decalcifying period. It was reduced to one third of the control in this study.

关 键 词：超声辐射 骨组织 脱钙处理 电镜检查 

分 类 号：R446.8[医药卫生—诊断学]