大鼠囊泡膜谷氨酸转运体样和谷氨酸脱羧酶样阳性终末与表达GABA_A受体α3亚单位的三叉神经中脑核神经元之间的联系(英文)  

CONNECTIONS BET WEEN VESICULAR GLUTAMATE TRANSPORTERS-,GAD-LIKE IMMUNOREACTIVE TERMINALS AND GABA_A RECEPTOR α3 SUBUNIT- LIKE POSITIVE NEURONS IN MESENCEPHALIC TRIGEMINAL NUCLEUS OF THE RAT

作　　者：陈鹏[1] 李金莲[1] 

机构地区：[1]第四军医大学解剖学教研室暨梁琚脑研究中心,西安710032

出　　处：《解剖学报》2003年第6期578-582,共5页Acta Anatomica Sinica

基　　金：FoundationforUniversityKeyTeacherbytheMinistryofEducation

摘　　要：目的　观察大鼠三叉神经中脑核 (Vme)内囊泡膜谷氨酸转运体 (VGluT1 和DNPI)样和谷氨酸脱羧酶 (GAD)样阳性终末与GABAA 受体α3亚单位 (GABAARα3)样阳性神经元之间的联系。　方法　免疫荧光组织化学三重染色技术 ,在激光共聚焦显微镜下观察。　结果　在Vme吻尾方向上 ,有大量的神经元胞体呈GABAARα3样免疫阳性 ,这些神经元多为大的假单极神经元 (直径为 2 5～ 5 0 μm)。VGluT1 样、DNPI样和GAD样免疫阳性终末密集分布于Vme内 ,一些VGluT1 DNPI样和GAD样阳性终末包绕在GABAARα3样阳性Vme神经元胞体周围 ,并与之形成密切接触。　结论　Vme神经元在介导口面部本体感觉信息的传递过程中 ,可能同时接受中枢其他来源的谷氨酸能和GABA能终末的调控 ,其中GABA能终末的作用可能是通过GABAA 受体实现的。Objective To observe the association of vesicular glutamate transporter of typeⅠ (VGluT1 ) like immunoreactive(LI) ,the differentiation-associated Na+ dependent inorganic phosphate cotransporter(DNPI) LI and glutami cacid decarboxy lase(GAD) LI terminals with GABAA receptor α3 subunit(GABAAR α3) LI neurons in mesencephalic trige minal nucleus of the rat.Methods Triple immun of luorescencehis to chemical staining technique and confo call aser scanning micros copy were used.Results Alargenumber of neuronal cell bodies showed GABAA Rα3 LI immunoreactivity atallrostrocaudal level softhe Vme ,and most of GABAAR α3 LI cells were large (2 5 5 0 μm)pseudouni polarneurons.The dense VGluT1 LI,DNPL LI and GAD LIterminal sdistri buted widelyin Vme ,some VGlu T1 DNPI LI and GAD LIterminals surrounded the somata of the GABAAR α3 LI Vmeneurons ,and made close contacts with them .Conclusion Proprioceptive sensory signals from the or of acialregionmight be modulated at the level of the primary afferent cell bodies in the Vme both by glutamatergic and GABAergic axonal terminals from other brain areas,and the effect of GABAergic terminals might be mediated by post synaptical GABAA receptors .

关 键 词：三叉神经中脑核 囊泡膜谷氨酸转运体 谷氨酸脱羧酶 免疫荧光组织化学 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]