体外培养的神经干细胞球的超微结构  

ULTRASTRUCTURE OF NEURAL STEM CELL NEUROSPHERE CULTURED in vitro

作　　者：郑志竑[1] 胡建石[1] 陈文列[2] 林玲[1] 钟秀容[2] 林建银[1] 

机构地区：[1]福建医科大学分子医学研究中心,福州350004 [2]福建医科大学电镜室,福州350004

出　　处：《解剖学报》2003年第6期615-619,共5页Acta Anatomica Sinica

基　　金：福建省科技计划重大项目 (2 0 0 1Z0 2 0 )

摘　　要：目的　探讨体外培养的神经干细胞球的超微结构。　方法　采用常规透射电镜结合硝酸镧示踪染色、红染色和单宁酸染色等技术 ,观察体外培养的大鼠脑神经干细胞球的超微结构。　结果　神经球中细胞之间连接较为松散 ,不存在紧密连接结构 ,神经干细胞增殖活跃 ,并可见神经球中有部分干细胞分化为具有突起和轴突样结构的细胞 ,甚至出现髓鞘样结构。　结论　揭示了体外培养的神经干细胞球的超微结构。Objective To study the ultrastructure of neural stem cell neurosphere cultured in vitro. Methods Neural stem cell neurospheres from the rat brain were observed under transmission electron microscope or with stain of lanthanum nitrate, ruthenium red and tannic acid. Results The conjunction between the cells within neurosphere was loose and no tight junction was observed. Neural stem cells were proliferating lively. Some neural stem cells differentiated into cells with process and structure of axon, even showed the structures similar to myelin.Conclusion The ultrastructure of neural stem cell neurosphere cultured in vitro was revealed.

关 键 词：神经干细胞 细胞球 体外培养 超微结构 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]