人胎儿睾丸发生过程中表皮生长因子与细胞增殖核抗原的表达  

EXPRESSION OF EGF AND PCNA DURING THE DEVELOPMENT OF HUMAN FETAL TESTIS

作　　者：牛嗣云[1] 高福禄[1] 庞小静[1] 李秀华[1] 

机构地区：[1]承德医学院中心实验室,承德067000

出　　处：《解剖学报》2003年第6期645-648,共4页Acta Anatomica Sinica

基　　金：河北省教育厅资助项目 (2 0 0 2 161)

摘　　要：目的　观察表皮生长因子 (EGF)与细胞增殖核抗原 (PCNA)在人胎儿睾丸发生过程中的表达特征 ,探讨两者在睾丸发生中的作用。　方法　用SP免疫细胞化学技术检测了人胎儿 12～ 32周睾丸间质细胞、支持细胞、生殖细胞EGF、PCNA阳性细胞表达率。　结果　EGF在胎儿 12周龄睾丸组织未见表达 ,16～ 32周龄睾丸间质细胞呈阳性表达 ,16～ 2 0周为表达高峰 ,随着胎龄的增长呈逐渐下降趋势 (P <0 0 1) ;PCNA在胎儿睾丸间质细胞、支持细胞和生殖细胞均有不同程度表达 ,16～ 2 0周为表达高峰 ,随着胎龄的增长呈逐渐下降趋势 (P <0 0 1)。　结论　EGF与PCNA在人胎儿睾丸发育过程中有不同程度的表达 。Objective To observe the expressing features of EGF and PCNA and study their roles during the development of human fetal testis. Methods The positive rates of EGF and PCNA in Leydig cells,Sertoli cells and germ cells of human fetal testis from 12th to 32th week were examined by SP immunocytochemical technique. Results The positive cells of EGF were not found in 12th week,and were found in Leydig cells from 16th to 32th week,the peak of expression was observed in 16th week.The positive staining was orange or brown and were scattered within the cytoplasm,the number of positive cells decreased gradually with the increasing of the fetal ages (P<0 01);Different degree expressions of PCNA were found in Leydig cells,Sertoli cells and Germ cells.The peak of expression was observed from 16th to 20th week.Positive nuclei were orange or brown.The number of positive cells decreased gradually with the increasing of the fetal ages (P<0 01).Conclusion Different degree expressions of EGF and PCNA were found during the development of human fetal testis;they have important roles during the testis development.

关 键 词：胎儿 睾丸发生 表皮生长因子 增殖细胞核抗原 免疫细胞化学 

分 类 号：R714.51[医药卫生—妇产科学] R321[医药卫生—临床医学]