人胎脑发育早期甲状腺激素受体mRNA的表达  

EXPRESSION OF THYROID HORMONE RECEPTOR mRNA DURING THE HUMAN BRAIN DEVELOPMENT

作　　者：刘春蓉[1] 李兰英[1] 刘奔[1] 臧晓怡[1] 苗军[2] 陈祖培[1] 

机构地区：[1]天津医科大学内分泌研究所,天津300070 [2]解放军总医院骨科研究所,北京100853

出　　处：《解剖学报》2003年第6期667-670,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 (3 0 2 3 0 3 3 0);天津市自然科学基金资助项目(0 13 611711)

摘　　要：目的　动态观察甲状腺激素受体 (TRs)mRNA在人脑发育过程中的表达变化。　方法　用RT PCR半定量法检测 3、5月龄人胎各脑区TRsmRNA表达变化。　结果　 3、5月龄人胎大脑、小脑、海马、丘脑、脑干及脊髓均有TRsmRNA表达 ,其中 3月龄胎脑各脑区TRsmRNA表达以TRα1 、TRα2 为主 ,TRβ1 次之。 5月龄胎脑各脑区TRsmRNA表达中以TRβ1 、α2 为主 ,TRα1 次之。TRsmRNA在大脑、小脑、海马处表达相对丰富。实验中发现的与TRα2始终伴行的特异条带经测序证实 ,除在 371 4 12位氨基酸处比TRα2 少 4 2个氨基酸 ,余序列两者完全相同。　结论　在脑发育过程中 ,TRsmRNA呈时空性表达 ,参与调节CNS的分化、成熟。首次验证了人TRα3与TRα2 编码区的差异序列。Objective To observe the variations of thyroid hormone receptors(RTs) mRNA experession during the human brain devlopment. Methods We investigated the ontogeny of TR isoforms in the first and second trimester human fetal different brain areas by semi-quantitative reverse transcriptase-polymerase chain reaction analysis. When we amplified the TRα 2 by PCR, the other sequence was amplified at the same time, it is about 100pb less than the RTα 2, so we cloned it into pGEM-T easy vector to determine its sequence. Results In the first and second trimester human fatal brain, RTs mRNAs were detected in cerebrum, cerebellum, brain, stem, hippocampus, spinal cord, thalamus. TRs mRNAs were relatively higher in cerebrum, cerebellum, hippocampus. In the first trimester human fatal brain, the TRα isoforms mRNAs were higher than TRβ 1, In the second trimester human fatal brain, the TRα 2 and TRβ 1 were higher than TRα 1. An additional truncated species was detected with the TRα 2 primer set. We submitted its sequencing results to Genbank, comparing it with TRα 2 by BLAST software, the results showed that it is identical to TRα 2 with the exception that it is missing 42 amino acids at 371-412 of TRα 2 sequence, so it is the human TRα 3. At the same time we acquired the Genbank accession number AF522368. Conclusion The spatial and temporal expressions of TR isoforms mRNAs exist in CNS development. We firstly assure the different sequence between human TRα2 and TRα3.

关 键 词：胎儿 脑发育 甲状腺激素受体 逆转录-聚合酶链反应 

分 类 号：R714.51[医药卫生—妇产科学]