低能量氦氖激光照射增加大鼠心肌血管内皮生长因子的表达  

LOW-POWER HELIUM-NEON LASER IRRADIATION ENHANCES EXPRESSION OF MYOCARDIAL VEGF OF THE RAT

作　　者：张卫光[1] 夏家骝[1] 田珑[1] 张书永[1] 

机构地区：[1]北京大学医学部解剖学与组织胚胎学系,北京100083

出　　处：《解剖学报》2003年第6期642-644,共3页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 (3 9470 3 63 )

摘　　要：目的　探求低能量氦氖激光照射大鼠心前区 ,对心肌血管内皮生长因子 (VEGF)表达的影响。　方法　采用RT PCR和免疫组织化学染色图像分析的方法 ,实验动物分对照组和激光照射组 ,每组 8只 ,照射组的照射剂量为 6 0 5J cm2 。　结果　VEGF免疫组织化学染色示照射组心肌微血管内皮细胞的着色较重 ,其平均光密度值为 0 2 4 6± 0 0 15 ,明显高于对照组的 0 2 18± 0 0 12 (t=9 0 5 ,P <0 0 5 ) ;心肌组织VEGF16 5的RT PCR产物表达显示 ,激光照射组的VEGF16 5mRNA表达为对照组的 2 79倍。　结论　以剂量为 6 0 5J cm2 的He Ne激光照射大鼠心前区 ,心肌微血管及心肌组织VEGF的表达明显增强。Objective To determine the effect of low power He Ne laser irradiation on expression of myocardial VEGF in precordial region of wistar rat. Methods VEGF expression in myocardium was studied by immunohistochemical methods,RT PCR and computer image analysis.Sixteen rats were divided equally into control and irradiated groups.He Ne laser (632 8?nm)was applied with the dose of 60 5?J/cm 2 in the irradiated group. Results The stain of VEGF immunohistochemistry of myocardium in irradiated group was stronger than that of in control group.The optic density in irradiated group(0 246±0 015)was higher than that of control group(0 218±0 012,P<0 05).The irradiated rats were 2 79 times the RT PCR products of VEGF 165 mRNA from myocardium of control rats.Conclusion Our studies demonstrate that He Ne laser irradiation (dose of 60 5?J/cm 2) increases productions of VEGF in myocardial micro vessels and myocardium.

关 键 词：低能量氦氖激光 心血管疾病 血管内皮生长因子 免疫组织化学 逆转录-聚合酶链反应 

分 类 号：R541[医药卫生—心血管疾病] R454.2[医药卫生—内科学]