小鼠前胃癌变粘膜中EGFR,cyclin D1,VEGF表达的相互关系  

Expression and interrelation of EGFR, cyclin D1, VEGF in mice model of forestomach carcinomatous mucosa

作　　者：周斌[1] 胡晓莺[2] 张潍[1] 李少民[1] 曹克宏[1] 付琨[1] 

机构地区：[1]西安交通大学第二医院胸心外科,陕西西安710004 [2]解放军401医院外科,山东青岛266071

出　　处：《第四军医大学学报》2003年第11期990-992,共3页Journal of the Fourth Military Medical University

基　　金：陕西省自然科学基金 (99SM59)

摘　　要：目的 :建立小鼠前胃癌变模型 ,揭示在前胃黏膜组织癌变前后表皮生长因子受体 (EGFR)、细胞周期素D1 (cyclinD1 )及血管内皮生长因子 (VEGF)表达水平的变化规律及相互关系 ,阐明食管癌及其癌前病变发生、发展的分子学机制 ,为食管癌高危人群的预防和治疗提供实验依据 .方法 :将 6 0只ICR雌性小鼠随机分为 2组 ,A组 :诱癌组 (n =4 0 ) ;用 2g·kg-1 肌氨酸乙酯盐酸盐和 0 .3g·kg-1 亚硝酸钠分别与 0 .0 1mol·L-1 盐酸配成 2 0 0g·L-1 和 30g·L-1 水溶液 ,灌喂前等量混合 ,每周灌喂 2次 ;B组 :对照组 (n =2 0 ) ,仅服用等量的生理盐水 .77d将小鼠全部处死 ,取前胃组织每例标本常规作一张HE染色以确定病变程度 ,免疫组化染色结果的判定以已知的皮肤EGFR、乳腺癌cyclinD1及血管瘤VEGF阳性切片作为阳性对照 ;应用 χ2 检验、非参数秩和检验及确切概率法对所得结果进行统计分析 .结果 :A组 1 0 0 %出现前胃上皮角化亢进 ,87.2 %有癌前期病变发生 ,癌总发生率为 35 .9% .癌变组织中只有EGFR与VEGF在程度进展上有密切关系 (χ2 =1 7.4 4 ,0 .0 2 5 <P <0 .0 5 ) ,二者呈正向变化 ,统计学意义显著 .结论 :EGFR ,cyclinD1 ,VEGF在癌前病变中表达水平均有显著提高 ,说明与癌肿的发生密切相关 ;而表达程度与组织?AIM: To reveal the regular pattern and interrelation of expression levels in EGFR (epidermal growth factor receptor), cyclin D1 (hormone of cell cycle D1) and VEGF (vascular endothelial growth factor), to clarify the molecule mechanism of occurrence and development in carcinoma of esophagus and precancerous lesion and to provide experimental evidence for the prevention and therapy of esophagus carcinoma in high risk group by establishing models of forestomach carcinomatous changes in mice. METHODS: 60 ICR female mice were divided into 2 groups at random. Group A: 60 ICR female mice were divided to 2 groups at random. Group A: carcinoma induced group,in which mice were fed twice a week with an equivalence mixture of aqueous solution of 200 g·L -1 sarcosine ester hydrochloride and 30 g·L -1 sodium nitrite and 0.01 mol·L -1 hydrochloric acid. Group B: Control group, in which mice were fed only with equivalence saline. All mice were sacrificed at the 77 day and their forestomach mucosas were routinely HE stained to decide the degree of lesion for each sample. The results of the immunity histochemistry staining were compared with the positive section of skin, mammary cancer and angioma. Statistical analysis of the results was made by χ 2 test, none parameter rank and summing test and precise probability. RESULTS: Gastric epithelium cornification excess in found in group A was 100%, precancerous lesion 87.2%, and the incidence of carcinoma 35.9%. In carcinoma tissues, only EGFR and VEGF had a close relationship in the degree and progress of lesion, with a positive correlation and statistical significance. CONCLUSION: Expression levels of EGFR, cyclin D1 and VEGF rise significantly in precancerous lesion, indicating that they have a close relationship with carcinogenesis, but they have less relationship with the histological classification. The rise of EGFR expression increases with the rise of VEGF level.

关 键 词：前胃癌 癌前病变 分子机制 

分 类 号：R392.13[医药卫生—免疫学]