Improvement of Copper-inducible Gene Expression System for Plant  

用于植物的铜诱导基因表达系统的改进(英文)

作　　者：彭向雷[1] 钟瑾[1] 梁斌[1] 胡鸢雷[1] 高音[1] 林忠平[1] 

机构地区：[1]北京大学生命科学学院蛋白质工程及植物基因工程国家重点实验室,北京100871

出　　处：《Acta Botanica Sinica》2003年第11期1307-1311,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家高技术研究和发展计划(863)项目(2001AA212161:2002AA224011)

摘　　要：The copper-regulated gene expression system has been developed to control spacial and temporal expression of transgene in plant. It comprises two parts: (1) ace I gene encoding copper-responsive transcription factor under the control of a constitutive or organ-specific promoter, and (2) a gene of interest under the control of a chimeric promoter consisting of the CaMV 35S (-90 to +8) promoter linked to the metal responsive element (MRE) carrying activating copper-metallothionein expression (ACE1)-binding sites. Here, the effectiveness of two different ACE1-binding cis -elements which derive from 5'-regulatory region of yeast metallothionein gene was investigated in transgenic tobacco (Nicotiana tabacum L. cv. W38). The results revealed that the MRE (-210 to -126) could increase the system inducibility by 50% - 100% compared with the previously reported MRE (-148 to -105). It is potential to use the copper-inducible system to control valuable gene traits in plant biotechnology.铜诱导基因表达系统已被用于植物转基因表达的时空调控。它由两部分构成:(1)组成型或器官专一性表达的acel基因编码铜反应性的转录因子;(2)融合启动子控制下的目的基因,融合启动子由含ACE1结合位点的金属反应元件(MRE)连接CaMV 35S(-90-+8)启动子组成。本实验测试了来自酵母金属硫蛋白基因5',调控区的两个不同的ACE1结合区域在转基因烟草(Nicotiana tabacum L.cv.W38)中的效果。结果表明,与MRE(-148--105)相比,使用MRE(-210--126)的铜诱导系统效率增加50％到100％。在植物生物技术中使用这一系统控制基因性状是很有潜力的。

关 键 词：copper-inducible system metal responsive element metal responsive transcription factor transgenic tobacco 

分 类 号：Q943[生物学—植物学]