Programmed Cell Death During Secondary Xylem Differentiation in Eucommia ulmoides  

杜仲次生木质部分化过程中的细胞编程死亡(英文)

作　　者：曹静[1] 贺新强[1] 王雅清[1] 苏都莫日根[1] 崔克明[1] 

机构地区：[1]北京大学生命科学学院,北京100871

出　　处：《Acta Botanica Sinica》2003年第12期1465-1474,共10页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家重点基础研究发展规划项目(G1999016002);国家自然科学基金(30070614, 30170056, 30000009)~~

摘　　要：Programmed cell death (PCD) during secondary xylem differentiation in Eucommia ulmoides Oliv. was examined using electron microscopy and by investigation of DNA fragmentation and degradation of caspase-like proteases (CLPs). DNA ladders were detected in developing secondary xylem by gel electrophoresis. DNA fragmentation was further confirmed by using the TdT-mediated dUTP nick-end labeling (TUNEL) method. Western blotting analysis showed that CLPs (caspase-8- and caspase-3-like proteases) and PARP (poly (ADP-ribose) polymerase) were degraded during secondary xylem differentiation. The results thus indicated that secondary xylem differentiation in E ulmoides was a typical process of PCD and the degradation of CLPs might be a constitutive PCD event during secondary xylem differentiation.通过电子显微镜观察、DNA断裂检测及类似半胱氨酸蛋白酶(caspase-like proteases, CLPs)降解检测等技术,对杜仲(Eucommia ulmoides Oliv.)次生木质部分化过程的细胞编程死亡进行了研究。分化中的次生木质部细胞总DNA凝胶电泳检测到DNA ladder,并通过TUNEL检测进一步确定了DNA被降解。Western blot结果表明:caspase-8和caspase-3状蛋白酶(caspase-8- 和 caspase-3-like proteases,CLPs)及多聚ADP-核糖聚合酶(poly(ADP-ribose) polymerase,PARP)在次生木质部分化过程中被降解。这些研究结果表明,杜仲次生木质部的细胞分化是一个典型的编程性死亡(Programmed cell death,PCD)过程,CLPs可能参与了此过程。

关 键 词：caspase-like protease DNA fragmentation Eucommia ulmoides poly (ADP-ribose) polymerase programmed cell death secondary xylem differentiation 

分 类 号：Q942[生物学—植物学]