栝楼性别分化特异大分子标记物研究  

Studies on the specific macromolecular markers of sex differentiation in Thrichosanthes kirilowii Maxim

作　　者：于凤池[1] 孙耘子[1] 袁高峰[1] 汪俏梅[1] 

机构地区：[1]浙江大学蔬菜研究所,浙江杭州310029

出　　处：《浙江农业学报》2003年第6期332-335,共4页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(30000015);浙江省教育厅资助项目。

摘　　要：对栝楼雌、雄株不同器官进行过氧化物酶同工酶活性和电泳分析。结果表明,雌株的过氧化物酶同工酶活性高于雄株,且雌株叶片中有一条特异性的过氧化物酶同工酶谱带;对雌、雄株不同发育时期的花器官进行可溶性蛋白的SDS PAGE分析表明,雄株幼花芽中存在一种23.4kD的特异蛋白质,雄花中一种32.3kD的蛋白质在受精后消失。The peroxidase isoenzyme activity and eletrophoresis analysis of various organs of male and female Thrichosanthes kirilowii Maxim plants were studied. The results indicated that the peroxidase isoenzyme activity in female plants was higher than that in male plants,and there was a specific peroxidase isoenzyme band in the leaf of female plants. SDSPAGE analysis of soluble proteins in male and female flowers at various developmental stages shown that there was a specific 23.4 kD protein in male flower bud, and a 32.3 kD protein in male flower disappeared after fertilization.

关 键 词：栝楼 性别分化 大分子标记物 过氧化物酶同工酶 特异蛋白质 

分 类 号：Q945.6[生物学—植物学] S642[农业科学—蔬菜学]